Cargando…

Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia

Neisseria meningitidis is the causative agent of invasive meningococcal disease (IMD). The BEXSERO(®) vaccine which is used to prevent serogroup B disease is composed of four sub-capsular protein antigens supplemented with an outer membrane vesicle. Since the sub-capsular protein antigens are variab...

Descripción completa

Detalles Bibliográficos
Autores principales: Mowlaboccus, Shakeel, Perkins, Timothy T., Smith, Helen, Sloots, Theo, Tozer, Sarah, Prempeh, Lydia-Jessica, Tay, Chin Yen, Peters, Fanny, Speers, David, Keil, Anthony D., Kahler, Charlene M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927168/
https://www.ncbi.nlm.nih.gov/pubmed/27355628
http://dx.doi.org/10.1371/journal.pone.0158315
_version_ 1782440231246495744
author Mowlaboccus, Shakeel
Perkins, Timothy T.
Smith, Helen
Sloots, Theo
Tozer, Sarah
Prempeh, Lydia-Jessica
Tay, Chin Yen
Peters, Fanny
Speers, David
Keil, Anthony D.
Kahler, Charlene M.
author_facet Mowlaboccus, Shakeel
Perkins, Timothy T.
Smith, Helen
Sloots, Theo
Tozer, Sarah
Prempeh, Lydia-Jessica
Tay, Chin Yen
Peters, Fanny
Speers, David
Keil, Anthony D.
Kahler, Charlene M.
author_sort Mowlaboccus, Shakeel
collection PubMed
description Neisseria meningitidis is the causative agent of invasive meningococcal disease (IMD). The BEXSERO(®) vaccine which is used to prevent serogroup B disease is composed of four sub-capsular protein antigens supplemented with an outer membrane vesicle. Since the sub-capsular protein antigens are variably expressed and antigenically variable amongst meningococcal isolates, vaccine coverage can be estimated by the meningococcal antigen typing system (MATS) which measures the propensity of the strain to be killed by vaccinated sera. Whole genome sequencing (WGS) which identifies the alleles of the antigens that may be recognised by the antibody response could represent, in future, an alternative estimate of coverage. In this study, WGS of 278 meningococcal isolates responsible for 62% of IMD in Western Australia from 2000–2014 were analysed for association of genetic lineage (sequence type [ST], clonal complex [cc]) with BEXSERO(®) antigen sequence type (BAST) and MATS to predict the annual vaccine coverage. A hyper-endemic period of IMD between 2000–05 was caused by cc41/44 with the major sequence type of ST-146 which was not predicted by MATS or BAST to be covered by the vaccine. An increase in serogroup diversity was observed between 2010–14 with the emergence of cc11 serogroup W in the adolescent population and cc23 serogroup Y in the elderly. BASTs were statistically associated with clonal complex although individual antigens underwent antigenic drift from the major type. BAST and MATS predicted an annual range of 44–91% vaccine coverage. Periods of low vaccine coverage in years post-2005 were not a result of the resurgence of cc41/44:ST-146 but were characterised by increased diversity of clonal complexes expressing BASTs which were not predicted by MATS to be covered by the vaccine. The driving force behind the diversity of the clonal complex and BAST during these periods of low vaccine coverage is unknown, but could be due to immune selection and inter-strain competition with carriage of non-disease causing meningococci.
format Online
Article
Text
id pubmed-4927168
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49271682016-07-18 Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia Mowlaboccus, Shakeel Perkins, Timothy T. Smith, Helen Sloots, Theo Tozer, Sarah Prempeh, Lydia-Jessica Tay, Chin Yen Peters, Fanny Speers, David Keil, Anthony D. Kahler, Charlene M. PLoS One Research Article Neisseria meningitidis is the causative agent of invasive meningococcal disease (IMD). The BEXSERO(®) vaccine which is used to prevent serogroup B disease is composed of four sub-capsular protein antigens supplemented with an outer membrane vesicle. Since the sub-capsular protein antigens are variably expressed and antigenically variable amongst meningococcal isolates, vaccine coverage can be estimated by the meningococcal antigen typing system (MATS) which measures the propensity of the strain to be killed by vaccinated sera. Whole genome sequencing (WGS) which identifies the alleles of the antigens that may be recognised by the antibody response could represent, in future, an alternative estimate of coverage. In this study, WGS of 278 meningococcal isolates responsible for 62% of IMD in Western Australia from 2000–2014 were analysed for association of genetic lineage (sequence type [ST], clonal complex [cc]) with BEXSERO(®) antigen sequence type (BAST) and MATS to predict the annual vaccine coverage. A hyper-endemic period of IMD between 2000–05 was caused by cc41/44 with the major sequence type of ST-146 which was not predicted by MATS or BAST to be covered by the vaccine. An increase in serogroup diversity was observed between 2010–14 with the emergence of cc11 serogroup W in the adolescent population and cc23 serogroup Y in the elderly. BASTs were statistically associated with clonal complex although individual antigens underwent antigenic drift from the major type. BAST and MATS predicted an annual range of 44–91% vaccine coverage. Periods of low vaccine coverage in years post-2005 were not a result of the resurgence of cc41/44:ST-146 but were characterised by increased diversity of clonal complexes expressing BASTs which were not predicted by MATS to be covered by the vaccine. The driving force behind the diversity of the clonal complex and BAST during these periods of low vaccine coverage is unknown, but could be due to immune selection and inter-strain competition with carriage of non-disease causing meningococci. Public Library of Science 2016-06-29 /pmc/articles/PMC4927168/ /pubmed/27355628 http://dx.doi.org/10.1371/journal.pone.0158315 Text en © 2016 Mowlaboccus et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mowlaboccus, Shakeel
Perkins, Timothy T.
Smith, Helen
Sloots, Theo
Tozer, Sarah
Prempeh, Lydia-Jessica
Tay, Chin Yen
Peters, Fanny
Speers, David
Keil, Anthony D.
Kahler, Charlene M.
Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia
title Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia
title_full Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia
title_fullStr Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia
title_full_unstemmed Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia
title_short Temporal Changes in BEXSERO(®) Antigen Sequence Type Associated with Genetic Lineages of Neisseria meningitidis over a 15-Year Period in Western Australia
title_sort temporal changes in bexsero(®) antigen sequence type associated with genetic lineages of neisseria meningitidis over a 15-year period in western australia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927168/
https://www.ncbi.nlm.nih.gov/pubmed/27355628
http://dx.doi.org/10.1371/journal.pone.0158315
work_keys_str_mv AT mowlaboccusshakeel temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT perkinstimothyt temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT smithhelen temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT slootstheo temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT tozersarah temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT prempehlydiajessica temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT taychinyen temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT petersfanny temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT speersdavid temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT keilanthonyd temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia
AT kahlercharlenem temporalchangesinbexseroantigensequencetypeassociatedwithgeneticlineagesofneisseriameningitidisovera15yearperiodinwesternaustralia