Cargando…

Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects

In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. p...

Descripción completa

Detalles Bibliográficos
Autores principales: Azizoglu, Ugur, Ayvaz, Abdurrahman, Yılmaz, Semih, Karabörklü, Salih, Temizgul, Rıdvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927682/
https://www.ncbi.nlm.nih.gov/pubmed/27143037
http://dx.doi.org/10.1016/j.bjm.2016.04.011
_version_ 1782440302986919936
author Azizoglu, Ugur
Ayvaz, Abdurrahman
Yılmaz, Semih
Karabörklü, Salih
Temizgul, Rıdvan
author_facet Azizoglu, Ugur
Ayvaz, Abdurrahman
Yılmaz, Semih
Karabörklü, Salih
Temizgul, Rıdvan
author_sort Azizoglu, Ugur
collection PubMed
description In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl(2). Isopropyl β-d-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 μg g(−1) caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains’ spore–crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
format Online
Article
Text
id pubmed-4927682
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-49276822016-07-13 Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects Azizoglu, Ugur Ayvaz, Abdurrahman Yılmaz, Semih Karabörklü, Salih Temizgul, Rıdvan Braz J Microbiol Environmental Microbiology In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl(2). Isopropyl β-d-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 μg g(−1) caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains’ spore–crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths. Elsevier 2016-04-20 /pmc/articles/PMC4927682/ /pubmed/27143037 http://dx.doi.org/10.1016/j.bjm.2016.04.011 Text en © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Environmental Microbiology
Azizoglu, Ugur
Ayvaz, Abdurrahman
Yılmaz, Semih
Karabörklü, Salih
Temizgul, Rıdvan
Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
title Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
title_full Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
title_fullStr Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
title_full_unstemmed Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
title_short Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
title_sort expression of cry1ab gene from a novel bacillus thuringiensis strain sy49-1 active on pest insects
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927682/
https://www.ncbi.nlm.nih.gov/pubmed/27143037
http://dx.doi.org/10.1016/j.bjm.2016.04.011
work_keys_str_mv AT azizogluugur expressionofcry1abgenefromanovelbacillusthuringiensisstrainsy491activeonpestinsects
AT ayvazabdurrahman expressionofcry1abgenefromanovelbacillusthuringiensisstrainsy491activeonpestinsects
AT yılmazsemih expressionofcry1abgenefromanovelbacillusthuringiensisstrainsy491activeonpestinsects
AT karaborklusalih expressionofcry1abgenefromanovelbacillusthuringiensisstrainsy491activeonpestinsects
AT temizgulrıdvan expressionofcry1abgenefromanovelbacillusthuringiensisstrainsy491activeonpestinsects