Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of...

Descripción completa

Detalles Bibliográficos
Autores principales: Manjegowda, Mohan C., Gupta, Paridhi Singhal, Limaye, Anil M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927973/
https://www.ncbi.nlm.nih.gov/pubmed/27408914
http://dx.doi.org/10.1016/j.dib.2016.03.054
_version_ 1782440349588783104
author Manjegowda, Mohan C.
Gupta, Paridhi Singhal
Limaye, Anil M.
author_facet Manjegowda, Mohan C.
Gupta, Paridhi Singhal
Limaye, Anil M.
author_sort Manjegowda, Mohan C.
collection PubMed
description Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines.
format Online
Article
Text
id pubmed-4927973
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-49279732016-07-12 Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30 Manjegowda, Mohan C. Gupta, Paridhi Singhal Limaye, Anil M. Data Brief Data Article Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines. Elsevier 2016-03-19 /pmc/articles/PMC4927973/ /pubmed/27408914 http://dx.doi.org/10.1016/j.dib.2016.03.054 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Manjegowda, Mohan C.
Gupta, Paridhi Singhal
Limaye, Anil M.
Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
title Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
title_full Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
title_fullStr Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
title_full_unstemmed Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
title_short Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30
title_sort validation data of a rabbit antiserum and affinity purified polyclonal antibody against the n-terminus of human gpr30
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927973/
https://www.ncbi.nlm.nih.gov/pubmed/27408914
http://dx.doi.org/10.1016/j.dib.2016.03.054
work_keys_str_mv AT manjegowdamohanc validationdataofarabbitantiserumandaffinitypurifiedpolyclonalantibodyagainstthenterminusofhumangpr30
AT guptaparidhisinghal validationdataofarabbitantiserumandaffinitypurifiedpolyclonalantibodyagainstthenterminusofhumangpr30
AT limayeanilm validationdataofarabbitantiserumandaffinitypurifiedpolyclonalantibodyagainstthenterminusofhumangpr30

Ejemplares similares