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Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation

Methanogens have gained much attention for their metabolic product, methane, which could be an energy substitute but also contributes to the greenhouse effect. One factor that controls methane emission, reversible protein phosphorylation, is a crucial signaling switch, and phosphoproteomics has beco...

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Autores principales: Wu, Wan-Ling, Lai, Shu-Jung, Yang, Jhih-Tian, Chern, Jeffy, Liang, Suh-Yuen, Chou, Chi-Chi, Kuo, Chih-Horng, Lai, Mei-Chin, Wu, Shih-Hsiung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928046/
https://www.ncbi.nlm.nih.gov/pubmed/27357474
http://dx.doi.org/10.1038/srep29013
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author Wu, Wan-Ling
Lai, Shu-Jung
Yang, Jhih-Tian
Chern, Jeffy
Liang, Suh-Yuen
Chou, Chi-Chi
Kuo, Chih-Horng
Lai, Mei-Chin
Wu, Shih-Hsiung
author_facet Wu, Wan-Ling
Lai, Shu-Jung
Yang, Jhih-Tian
Chern, Jeffy
Liang, Suh-Yuen
Chou, Chi-Chi
Kuo, Chih-Horng
Lai, Mei-Chin
Wu, Shih-Hsiung
author_sort Wu, Wan-Ling
collection PubMed
description Methanogens have gained much attention for their metabolic product, methane, which could be an energy substitute but also contributes to the greenhouse effect. One factor that controls methane emission, reversible protein phosphorylation, is a crucial signaling switch, and phosphoproteomics has become a powerful tool for large-scale surveying. Here, we conducted the first phosphorylation-mediated regulation study in halophilic Methanohalophilus portucalensis FDF1(T), a model strain for studying stress response mechanisms in osmoadaptation. A shotgun approach and MS-based analysis identified 149 unique phosphoproteins. Among them, 26% participated in methanogenesis and osmolytes biosynthesis pathways. Of note, we uncovered that protein phosphorylation might be a crucial factor to modulate the pyrrolysine (Pyl) incorporation and Pyl-mediated methylotrophic methanogenesis. Furthermore, heterologous expression of glycine sarcosine N-methyltransferase (GSMT) mutant derivatives in the osmosensitive Escherichia coli MKH13 revealed that the nonphosphorylated T68A mutant resulted in increased salt tolerance. In contrast, mimic phosphorylated mutant T68D proved defective in both enzymatic activity and salinity tolerance for growth. Our study provides new insights into phosphorylation modification as a crucial role of both methanogenesis and osmoadaptation in methanoarchaea, promoting biogas production or reducing future methane emission in response to global warming and climate change.
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spelling pubmed-49280462016-07-01 Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation Wu, Wan-Ling Lai, Shu-Jung Yang, Jhih-Tian Chern, Jeffy Liang, Suh-Yuen Chou, Chi-Chi Kuo, Chih-Horng Lai, Mei-Chin Wu, Shih-Hsiung Sci Rep Article Methanogens have gained much attention for their metabolic product, methane, which could be an energy substitute but also contributes to the greenhouse effect. One factor that controls methane emission, reversible protein phosphorylation, is a crucial signaling switch, and phosphoproteomics has become a powerful tool for large-scale surveying. Here, we conducted the first phosphorylation-mediated regulation study in halophilic Methanohalophilus portucalensis FDF1(T), a model strain for studying stress response mechanisms in osmoadaptation. A shotgun approach and MS-based analysis identified 149 unique phosphoproteins. Among them, 26% participated in methanogenesis and osmolytes biosynthesis pathways. Of note, we uncovered that protein phosphorylation might be a crucial factor to modulate the pyrrolysine (Pyl) incorporation and Pyl-mediated methylotrophic methanogenesis. Furthermore, heterologous expression of glycine sarcosine N-methyltransferase (GSMT) mutant derivatives in the osmosensitive Escherichia coli MKH13 revealed that the nonphosphorylated T68A mutant resulted in increased salt tolerance. In contrast, mimic phosphorylated mutant T68D proved defective in both enzymatic activity and salinity tolerance for growth. Our study provides new insights into phosphorylation modification as a crucial role of both methanogenesis and osmoadaptation in methanoarchaea, promoting biogas production or reducing future methane emission in response to global warming and climate change. Nature Publishing Group 2016-06-30 /pmc/articles/PMC4928046/ /pubmed/27357474 http://dx.doi.org/10.1038/srep29013 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wu, Wan-Ling
Lai, Shu-Jung
Yang, Jhih-Tian
Chern, Jeffy
Liang, Suh-Yuen
Chou, Chi-Chi
Kuo, Chih-Horng
Lai, Mei-Chin
Wu, Shih-Hsiung
Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation
title Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation
title_full Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation
title_fullStr Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation
title_full_unstemmed Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation
title_short Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation
title_sort phosphoproteomic analysis of methanohalophilus portucalensis fdf1(t) identified the role of protein phosphorylation in methanogenesis and osmoregulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928046/
https://www.ncbi.nlm.nih.gov/pubmed/27357474
http://dx.doi.org/10.1038/srep29013
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