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Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system

The human body contains different endothelial cell types and differences in their angiogenic potential are poorly understood. We compared the functional angiogenic ability of human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using a three-dimensional (3D) mic...

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Autores principales: Seo, Ha-Rim, Jeong, Hyo Eun, Joo, Hyung Joon, Choi, Seung-Cheol, Park, Chi-Yeon, Kim, Jong-Ho, Choi, Ji-Hyun, Cui, Long-Hui, Hong, Soon Jun, Chung, Seok, Lim, Do-Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928073/
https://www.ncbi.nlm.nih.gov/pubmed/27357248
http://dx.doi.org/10.1038/srep28832
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author Seo, Ha-Rim
Jeong, Hyo Eun
Joo, Hyung Joon
Choi, Seung-Cheol
Park, Chi-Yeon
Kim, Jong-Ho
Choi, Ji-Hyun
Cui, Long-Hui
Hong, Soon Jun
Chung, Seok
Lim, Do-Sun
author_facet Seo, Ha-Rim
Jeong, Hyo Eun
Joo, Hyung Joon
Choi, Seung-Cheol
Park, Chi-Yeon
Kim, Jong-Ho
Choi, Ji-Hyun
Cui, Long-Hui
Hong, Soon Jun
Chung, Seok
Lim, Do-Sun
author_sort Seo, Ha-Rim
collection PubMed
description The human body contains different endothelial cell types and differences in their angiogenic potential are poorly understood. We compared the functional angiogenic ability of human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using a three-dimensional (3D) microfluidic cell culture system. HAECs and HUVECs exhibited similar cellular characteristics in a 2D culture system; however, in the 3D microfluidic angiogenesis system, HAECs exhibited stronger angiogenic potential than HUVECs. Interestingly, the expression level of fibroblast growth factor (FGF)2 and FGF5 under vascular endothelial growth factor (VEGF)-A stimulation was significantly higher in HAECs than in HUVECs. Moreover, small interfering RNA-mediated knockdown of FGF2 and FGF5 more significantly attenuated vascular sprouting induced from HAECs than HUVECs. Our results suggest that HAECs have greater angiogenic potential through FGF2 and FGF5 upregulation and could be a compatible endothelial cell type to achieve robust angiogenesis.
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spelling pubmed-49280732016-07-01 Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system Seo, Ha-Rim Jeong, Hyo Eun Joo, Hyung Joon Choi, Seung-Cheol Park, Chi-Yeon Kim, Jong-Ho Choi, Ji-Hyun Cui, Long-Hui Hong, Soon Jun Chung, Seok Lim, Do-Sun Sci Rep Article The human body contains different endothelial cell types and differences in their angiogenic potential are poorly understood. We compared the functional angiogenic ability of human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using a three-dimensional (3D) microfluidic cell culture system. HAECs and HUVECs exhibited similar cellular characteristics in a 2D culture system; however, in the 3D microfluidic angiogenesis system, HAECs exhibited stronger angiogenic potential than HUVECs. Interestingly, the expression level of fibroblast growth factor (FGF)2 and FGF5 under vascular endothelial growth factor (VEGF)-A stimulation was significantly higher in HAECs than in HUVECs. Moreover, small interfering RNA-mediated knockdown of FGF2 and FGF5 more significantly attenuated vascular sprouting induced from HAECs than HUVECs. Our results suggest that HAECs have greater angiogenic potential through FGF2 and FGF5 upregulation and could be a compatible endothelial cell type to achieve robust angiogenesis. Nature Publishing Group 2016-06-30 /pmc/articles/PMC4928073/ /pubmed/27357248 http://dx.doi.org/10.1038/srep28832 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Seo, Ha-Rim
Jeong, Hyo Eun
Joo, Hyung Joon
Choi, Seung-Cheol
Park, Chi-Yeon
Kim, Jong-Ho
Choi, Ji-Hyun
Cui, Long-Hui
Hong, Soon Jun
Chung, Seok
Lim, Do-Sun
Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system
title Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system
title_full Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system
title_fullStr Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system
title_full_unstemmed Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system
title_short Intrinsic FGF2 and FGF5 promotes angiogenesis of human aortic endothelial cells in 3D microfluidic angiogenesis system
title_sort intrinsic fgf2 and fgf5 promotes angiogenesis of human aortic endothelial cells in 3d microfluidic angiogenesis system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928073/
https://www.ncbi.nlm.nih.gov/pubmed/27357248
http://dx.doi.org/10.1038/srep28832
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