A fast and easy strategy for protein purification using “teabags”

Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method w...

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Detalles Bibliográficos
Autores principales: Castaldo, M., Barlind, L., Mauritzson, F., Wan, P. T., Snijder, H. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928122/
https://www.ncbi.nlm.nih.gov/pubmed/27356497
http://dx.doi.org/10.1038/srep28887
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author Castaldo, M.
Barlind, L.
Mauritzson, F.
Wan, P. T.
Snijder, H. J.
author_facet Castaldo, M.
Barlind, L.
Mauritzson, F.
Wan, P. T.
Snijder, H. J.
author_sort Castaldo, M.
collection PubMed
description Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method where affinity resin is contained within porous-walled containers, supporting clarification, product recovery, and concentration in a single step with minimal hands-on processing time, without significant investments in equipment.
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spelling pubmed-49281222016-07-01 A fast and easy strategy for protein purification using “teabags” Castaldo, M. Barlind, L. Mauritzson, F. Wan, P. T. Snijder, H. J. Sci Rep Article Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method where affinity resin is contained within porous-walled containers, supporting clarification, product recovery, and concentration in a single step with minimal hands-on processing time, without significant investments in equipment. Nature Publishing Group 2016-06-30 /pmc/articles/PMC4928122/ /pubmed/27356497 http://dx.doi.org/10.1038/srep28887 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Castaldo, M.
Barlind, L.
Mauritzson, F.
Wan, P. T.
Snijder, H. J.
A fast and easy strategy for protein purification using “teabags”
title A fast and easy strategy for protein purification using “teabags”
title_full A fast and easy strategy for protein purification using “teabags”
title_fullStr A fast and easy strategy for protein purification using “teabags”
title_full_unstemmed A fast and easy strategy for protein purification using “teabags”
title_short A fast and easy strategy for protein purification using “teabags”
title_sort fast and easy strategy for protein purification using “teabags”
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928122/
https://www.ncbi.nlm.nih.gov/pubmed/27356497
http://dx.doi.org/10.1038/srep28887
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