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A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti

BACKGROUND: The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only...

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Autores principales: Rahola, Nil, Henni, Leila Hadj, Obame, Judicael, Ayala, Diego, Makanga, Boris Kevin, Lehrter, Véronique, Izri, Arezki, Paupy, Christophe, Depaquit, Jérôme
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928309/
https://www.ncbi.nlm.nih.gov/pubmed/27357585
http://dx.doi.org/10.1186/s13071-016-1656-5
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author Rahola, Nil
Henni, Leila Hadj
Obame, Judicael
Ayala, Diego
Makanga, Boris Kevin
Lehrter, Véronique
Izri, Arezki
Paupy, Christophe
Depaquit, Jérôme
author_facet Rahola, Nil
Henni, Leila Hadj
Obame, Judicael
Ayala, Diego
Makanga, Boris Kevin
Lehrter, Véronique
Izri, Arezki
Paupy, Christophe
Depaquit, Jérôme
author_sort Rahola, Nil
collection PubMed
description BACKGROUND: The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only few morphological studies have addressed relationships between species of Spelaeomyia. METHODS: Specimens of Sa. moucheti were collected in two different sites in Gabon. Spelaeomyia emilii and Sa. darlingi specimens came from Gabon and Mali. Specimens of Sa. mirabilis were collected in the Democratic Republic of Congo and Gabon. All specimens were caught using CDC miniature light traps, then dissected, both heads and genitalia were kept for morphological analysis and the rest of the bodies were kept for molecular processing and analyses. RESULTS: Some unidentified males are associated to Sa. moucheti females using molecular tools and are described for the first time. A new morphological feature is observed on the spermathecae of the female and new drawings are provided. For the first time a phylogenetic analysis is carried out on rDNA and mtDNA markers and it shows that Sa. moucheti is the sister species of Sa. mirabilis. CONCLUSIONS: Spelaeomyia moucheti is the sister species of Sa. mirabilis. This result is in agreement with the sharing of morphological characters between these closely related species. Moreover, these two species are not as cavernicolous as literature previously indicated. They were caught in open rainforest in Gabon.
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spelling pubmed-49283092016-06-30 A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti Rahola, Nil Henni, Leila Hadj Obame, Judicael Ayala, Diego Makanga, Boris Kevin Lehrter, Véronique Izri, Arezki Paupy, Christophe Depaquit, Jérôme Parasit Vectors Research BACKGROUND: The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only few morphological studies have addressed relationships between species of Spelaeomyia. METHODS: Specimens of Sa. moucheti were collected in two different sites in Gabon. Spelaeomyia emilii and Sa. darlingi specimens came from Gabon and Mali. Specimens of Sa. mirabilis were collected in the Democratic Republic of Congo and Gabon. All specimens were caught using CDC miniature light traps, then dissected, both heads and genitalia were kept for morphological analysis and the rest of the bodies were kept for molecular processing and analyses. RESULTS: Some unidentified males are associated to Sa. moucheti females using molecular tools and are described for the first time. A new morphological feature is observed on the spermathecae of the female and new drawings are provided. For the first time a phylogenetic analysis is carried out on rDNA and mtDNA markers and it shows that Sa. moucheti is the sister species of Sa. mirabilis. CONCLUSIONS: Spelaeomyia moucheti is the sister species of Sa. mirabilis. This result is in agreement with the sharing of morphological characters between these closely related species. Moreover, these two species are not as cavernicolous as literature previously indicated. They were caught in open rainforest in Gabon. BioMed Central 2016-06-29 /pmc/articles/PMC4928309/ /pubmed/27357585 http://dx.doi.org/10.1186/s13071-016-1656-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Rahola, Nil
Henni, Leila Hadj
Obame, Judicael
Ayala, Diego
Makanga, Boris Kevin
Lehrter, Véronique
Izri, Arezki
Paupy, Christophe
Depaquit, Jérôme
A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
title A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
title_full A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
title_fullStr A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
title_full_unstemmed A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
title_short A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
title_sort molecular study of the genus spelaeomyia (diptera: phlebotominae) with description of the male of spelaeomyia moucheti
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928309/
https://www.ncbi.nlm.nih.gov/pubmed/27357585
http://dx.doi.org/10.1186/s13071-016-1656-5
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