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A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti
BACKGROUND: The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928309/ https://www.ncbi.nlm.nih.gov/pubmed/27357585 http://dx.doi.org/10.1186/s13071-016-1656-5 |
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author | Rahola, Nil Henni, Leila Hadj Obame, Judicael Ayala, Diego Makanga, Boris Kevin Lehrter, Véronique Izri, Arezki Paupy, Christophe Depaquit, Jérôme |
author_facet | Rahola, Nil Henni, Leila Hadj Obame, Judicael Ayala, Diego Makanga, Boris Kevin Lehrter, Véronique Izri, Arezki Paupy, Christophe Depaquit, Jérôme |
author_sort | Rahola, Nil |
collection | PubMed |
description | BACKGROUND: The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only few morphological studies have addressed relationships between species of Spelaeomyia. METHODS: Specimens of Sa. moucheti were collected in two different sites in Gabon. Spelaeomyia emilii and Sa. darlingi specimens came from Gabon and Mali. Specimens of Sa. mirabilis were collected in the Democratic Republic of Congo and Gabon. All specimens were caught using CDC miniature light traps, then dissected, both heads and genitalia were kept for morphological analysis and the rest of the bodies were kept for molecular processing and analyses. RESULTS: Some unidentified males are associated to Sa. moucheti females using molecular tools and are described for the first time. A new morphological feature is observed on the spermathecae of the female and new drawings are provided. For the first time a phylogenetic analysis is carried out on rDNA and mtDNA markers and it shows that Sa. moucheti is the sister species of Sa. mirabilis. CONCLUSIONS: Spelaeomyia moucheti is the sister species of Sa. mirabilis. This result is in agreement with the sharing of morphological characters between these closely related species. Moreover, these two species are not as cavernicolous as literature previously indicated. They were caught in open rainforest in Gabon. |
format | Online Article Text |
id | pubmed-4928309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49283092016-06-30 A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti Rahola, Nil Henni, Leila Hadj Obame, Judicael Ayala, Diego Makanga, Boris Kevin Lehrter, Véronique Izri, Arezki Paupy, Christophe Depaquit, Jérôme Parasit Vectors Research BACKGROUND: The genus Spelaeomyia includes four African species considered as being cavernicolous: Spelaeomyia darlingi, Spelaeomyia mirabilis, Spelaeomyia emilii and Spelaeomyia moucheti. Despite a potential role in Leishmania major leishmaniasis transmission in Mali, no molecular studies and only few morphological studies have addressed relationships between species of Spelaeomyia. METHODS: Specimens of Sa. moucheti were collected in two different sites in Gabon. Spelaeomyia emilii and Sa. darlingi specimens came from Gabon and Mali. Specimens of Sa. mirabilis were collected in the Democratic Republic of Congo and Gabon. All specimens were caught using CDC miniature light traps, then dissected, both heads and genitalia were kept for morphological analysis and the rest of the bodies were kept for molecular processing and analyses. RESULTS: Some unidentified males are associated to Sa. moucheti females using molecular tools and are described for the first time. A new morphological feature is observed on the spermathecae of the female and new drawings are provided. For the first time a phylogenetic analysis is carried out on rDNA and mtDNA markers and it shows that Sa. moucheti is the sister species of Sa. mirabilis. CONCLUSIONS: Spelaeomyia moucheti is the sister species of Sa. mirabilis. This result is in agreement with the sharing of morphological characters between these closely related species. Moreover, these two species are not as cavernicolous as literature previously indicated. They were caught in open rainforest in Gabon. BioMed Central 2016-06-29 /pmc/articles/PMC4928309/ /pubmed/27357585 http://dx.doi.org/10.1186/s13071-016-1656-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Rahola, Nil Henni, Leila Hadj Obame, Judicael Ayala, Diego Makanga, Boris Kevin Lehrter, Véronique Izri, Arezki Paupy, Christophe Depaquit, Jérôme A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti |
title | A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti |
title_full | A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti |
title_fullStr | A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti |
title_full_unstemmed | A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti |
title_short | A molecular study of the genus Spelaeomyia (Diptera: Phlebotominae) with description of the male of Spelaeomyia moucheti |
title_sort | molecular study of the genus spelaeomyia (diptera: phlebotominae) with description of the male of spelaeomyia moucheti |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928309/ https://www.ncbi.nlm.nih.gov/pubmed/27357585 http://dx.doi.org/10.1186/s13071-016-1656-5 |
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