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Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children

BACKGROUND: Plasmodium falciparum infection can lead to several clinical manifestations ranging from asymptomatic infections (AM) and uncomplicated malaria (UM) to potentially fatal severe malaria (SM), including cerebral malaria (CM). Factors implicated in the progression towards severe disease are...

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Autores principales: Tahar, Rachida, Albergaria, Catarina, Zeghidour, Neil, Ngane, Vincent Foumane, Basco, Leonardo K., Roussilhon, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928328/
https://www.ncbi.nlm.nih.gov/pubmed/27357958
http://dx.doi.org/10.1186/s12936-016-1378-3
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author Tahar, Rachida
Albergaria, Catarina
Zeghidour, Neil
Ngane, Vincent Foumane
Basco, Leonardo K.
Roussilhon, Christian
author_facet Tahar, Rachida
Albergaria, Catarina
Zeghidour, Neil
Ngane, Vincent Foumane
Basco, Leonardo K.
Roussilhon, Christian
author_sort Tahar, Rachida
collection PubMed
description BACKGROUND: Plasmodium falciparum infection can lead to several clinical manifestations ranging from asymptomatic infections (AM) and uncomplicated malaria (UM) to potentially fatal severe malaria (SM), including cerebral malaria (CM). Factors implicated in the progression towards severe disease are not fully understood. METHODS: In the present study, an enzyme-linked immunosorbent assay (ELISA) method was used to investigate the plasma content of several biomarkers of the immune response, namely Neopterin, sCD163, suPAR, Pentraxin 3 (PTX3), sCD14, Fractalkine (CX3CL1), sTREM-1 and MIG (CXCL9), in patients with distinct clinical manifestations of malaria. The goal of this study was to determine the relative involvement of these inflammatory mediators in the pathogenesis of malaria and test their relevance as biomarkers of disease severity. RESULTS: ROC curve analysis show that children with AM were characterized by high levels of Fractalkine and sCD163 whereas children with UM were distinguishable by the presence of PTX3 in their plasma. Furthermore, principal component analysis indicated that the combination of Fractalkine, MIG, and Neopterin was the best predictor of AM condition, while suPAR, PTX3 and sTREM-1 combination was the best indicator of UM when compared to AM. The association of Neopterin, suPAR and Fractalkine was strongly predictive of SM or CM compared to UM. CONCLUSIONS: The results indicate that the simultaneous evaluation of these bioactive molecules as quantifiable blood parameters may be helpful to get a better insight into the clinical syndromes in children with malaria.
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spelling pubmed-49283282016-06-30 Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children Tahar, Rachida Albergaria, Catarina Zeghidour, Neil Ngane, Vincent Foumane Basco, Leonardo K. Roussilhon, Christian Malar J Research BACKGROUND: Plasmodium falciparum infection can lead to several clinical manifestations ranging from asymptomatic infections (AM) and uncomplicated malaria (UM) to potentially fatal severe malaria (SM), including cerebral malaria (CM). Factors implicated in the progression towards severe disease are not fully understood. METHODS: In the present study, an enzyme-linked immunosorbent assay (ELISA) method was used to investigate the plasma content of several biomarkers of the immune response, namely Neopterin, sCD163, suPAR, Pentraxin 3 (PTX3), sCD14, Fractalkine (CX3CL1), sTREM-1 and MIG (CXCL9), in patients with distinct clinical manifestations of malaria. The goal of this study was to determine the relative involvement of these inflammatory mediators in the pathogenesis of malaria and test their relevance as biomarkers of disease severity. RESULTS: ROC curve analysis show that children with AM were characterized by high levels of Fractalkine and sCD163 whereas children with UM were distinguishable by the presence of PTX3 in their plasma. Furthermore, principal component analysis indicated that the combination of Fractalkine, MIG, and Neopterin was the best predictor of AM condition, while suPAR, PTX3 and sTREM-1 combination was the best indicator of UM when compared to AM. The association of Neopterin, suPAR and Fractalkine was strongly predictive of SM or CM compared to UM. CONCLUSIONS: The results indicate that the simultaneous evaluation of these bioactive molecules as quantifiable blood parameters may be helpful to get a better insight into the clinical syndromes in children with malaria. BioMed Central 2016-06-29 /pmc/articles/PMC4928328/ /pubmed/27357958 http://dx.doi.org/10.1186/s12936-016-1378-3 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Tahar, Rachida
Albergaria, Catarina
Zeghidour, Neil
Ngane, Vincent Foumane
Basco, Leonardo K.
Roussilhon, Christian
Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
title Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
title_full Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
title_fullStr Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
title_full_unstemmed Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
title_short Plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in African children
title_sort plasma levels of eight different mediators and their potential as biomarkers of various clinical malaria conditions in african children
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928328/
https://www.ncbi.nlm.nih.gov/pubmed/27357958
http://dx.doi.org/10.1186/s12936-016-1378-3
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