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Simplified Bryostatin Analogues Protect Cells from Chikungunya Virus-Induced Cell Death
[Image: see text] Chikungunya virus (CHIKV) is a mosquito-borne alphavirus showing a recent resurgence and rapid spread worldwide. While vaccines are under development, there are currently no therapies to treat this disease, except for over-the-counter (OTC) analgesics, which alleviate the devastati...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society and American Society of Pharmacognosy
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928627/ https://www.ncbi.nlm.nih.gov/pubmed/26900625 http://dx.doi.org/10.1021/acs.jnatprod.5b01016 |
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author | Staveness, Daryl Abdelnabi, Rana Schrier, Adam J. Loy, Brian A. Verma, Vishal A. DeChristopher, Brian A. Near, Katherine E. Neyts, Johan Delang, Leen Leyssen, Pieter Wender, Paul A. |
author_facet | Staveness, Daryl Abdelnabi, Rana Schrier, Adam J. Loy, Brian A. Verma, Vishal A. DeChristopher, Brian A. Near, Katherine E. Neyts, Johan Delang, Leen Leyssen, Pieter Wender, Paul A. |
author_sort | Staveness, Daryl |
collection | PubMed |
description | [Image: see text] Chikungunya virus (CHIKV) is a mosquito-borne alphavirus showing a recent resurgence and rapid spread worldwide. While vaccines are under development, there are currently no therapies to treat this disease, except for over-the-counter (OTC) analgesics, which alleviate the devastating arthritic and arthralgic symptoms. To identify novel inhibitors of the virus, analogues of the natural product bryostatin 1, a clinical lead for the treatment of cancer, Alzheimer’s disease, and HIV eradication, were investigated for in vitro antiviral activity and were found to be among the most potent inhibitors of CHIKV replication reported to date. Bryostatin-based therapeutic efforts and even recent anti-CHIKV strategies have centered on modulation of protein kinase C (PKC). Intriguingly, while the C ring of bryostatin primarily drives interactions with PKC, A- and B-ring functionality in these analogues has a significant effect on the observed cell-protective activity. Significantly, bryostatin 1 itself, a potent pan-PKC modulator, is inactive in these assays. These new findings indicate that the observed anti-CHIKV activity is not solely mediated by PKC modulation, suggesting possible as yet unidentified targets for CHIKV therapeutic intervention. The high potency and low toxicity of these bryologs make them promising new leads for the development of a CHIKV treatment. |
format | Online Article Text |
id | pubmed-4928627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Chemical
Society and American Society of Pharmacognosy |
record_format | MEDLINE/PubMed |
spelling | pubmed-49286272017-02-22 Simplified Bryostatin Analogues Protect Cells from Chikungunya Virus-Induced Cell Death Staveness, Daryl Abdelnabi, Rana Schrier, Adam J. Loy, Brian A. Verma, Vishal A. DeChristopher, Brian A. Near, Katherine E. Neyts, Johan Delang, Leen Leyssen, Pieter Wender, Paul A. J Nat Prod [Image: see text] Chikungunya virus (CHIKV) is a mosquito-borne alphavirus showing a recent resurgence and rapid spread worldwide. While vaccines are under development, there are currently no therapies to treat this disease, except for over-the-counter (OTC) analgesics, which alleviate the devastating arthritic and arthralgic symptoms. To identify novel inhibitors of the virus, analogues of the natural product bryostatin 1, a clinical lead for the treatment of cancer, Alzheimer’s disease, and HIV eradication, were investigated for in vitro antiviral activity and were found to be among the most potent inhibitors of CHIKV replication reported to date. Bryostatin-based therapeutic efforts and even recent anti-CHIKV strategies have centered on modulation of protein kinase C (PKC). Intriguingly, while the C ring of bryostatin primarily drives interactions with PKC, A- and B-ring functionality in these analogues has a significant effect on the observed cell-protective activity. Significantly, bryostatin 1 itself, a potent pan-PKC modulator, is inactive in these assays. These new findings indicate that the observed anti-CHIKV activity is not solely mediated by PKC modulation, suggesting possible as yet unidentified targets for CHIKV therapeutic intervention. The high potency and low toxicity of these bryologs make them promising new leads for the development of a CHIKV treatment. American Chemical Society and American Society of Pharmacognosy 2016-02-22 2016-04-22 /pmc/articles/PMC4928627/ /pubmed/26900625 http://dx.doi.org/10.1021/acs.jnatprod.5b01016 Text en Copyright © 2016 American Chemical Society and American Society of Pharmacognosy This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Staveness, Daryl Abdelnabi, Rana Schrier, Adam J. Loy, Brian A. Verma, Vishal A. DeChristopher, Brian A. Near, Katherine E. Neyts, Johan Delang, Leen Leyssen, Pieter Wender, Paul A. Simplified Bryostatin Analogues Protect Cells from Chikungunya Virus-Induced Cell Death |
title | Simplified Bryostatin Analogues Protect Cells from
Chikungunya Virus-Induced Cell Death |
title_full | Simplified Bryostatin Analogues Protect Cells from
Chikungunya Virus-Induced Cell Death |
title_fullStr | Simplified Bryostatin Analogues Protect Cells from
Chikungunya Virus-Induced Cell Death |
title_full_unstemmed | Simplified Bryostatin Analogues Protect Cells from
Chikungunya Virus-Induced Cell Death |
title_short | Simplified Bryostatin Analogues Protect Cells from
Chikungunya Virus-Induced Cell Death |
title_sort | simplified bryostatin analogues protect cells from
chikungunya virus-induced cell death |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928627/ https://www.ncbi.nlm.nih.gov/pubmed/26900625 http://dx.doi.org/10.1021/acs.jnatprod.5b01016 |
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