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Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition

Kiwifruit allergy has been described mostly in the adult population, but immunoglobulin (Ig)E-mediated allergic reactions to kiwifruit appear to be occurring more frequently in children. To date, 13 allergens from kiwifruit have been identified. Our aim was to identify kiwifruit allergens in a kiwif...

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Autores principales: Moreno Álvarez, Ana, Vila Sexto, Leticia, Bardina, Luda, Grishina, Galina, Sampson, Hugh. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928771/
https://www.ncbi.nlm.nih.gov/pubmed/27417374
http://dx.doi.org/10.3390/children2040424
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author Moreno Álvarez, Ana
Vila Sexto, Leticia
Bardina, Luda
Grishina, Galina
Sampson, Hugh. A.
author_facet Moreno Álvarez, Ana
Vila Sexto, Leticia
Bardina, Luda
Grishina, Galina
Sampson, Hugh. A.
author_sort Moreno Álvarez, Ana
collection PubMed
description Kiwifruit allergy has been described mostly in the adult population, but immunoglobulin (Ig)E-mediated allergic reactions to kiwifruit appear to be occurring more frequently in children. To date, 13 allergens from kiwifruit have been identified. Our aim was to identify kiwifruit allergens in a kiwifruit allergic-pediatric population, describing clinical manifestations and patterns of recognition. Twenty-four children were included. Diagnosis of kiwifruit allergy was based on compatible clinical manifestations and demonstration of specific IgE by skin prick test (SPT) and/or serum-specific IgE determination. SDS-PAGE and immunoblotting were performed with kiwifruit extract, and proteins of interest were further analyzed by mass spectrometry/mass spectrometry. For component-resolved in vitro diagnosis, sera of kiwifruit-allergic patients were analyzed by an allergen microarray assay. Act d 1 and Act d 2 were bound by IgE from 15 of 24 children. Two children with systemic manifestations recognized a protein of 15 kDa, homologous to Act d 5. Act d 1 was the allergen with the highest frequency of recognition on microarray chip, followed by Act d 2 and Act d 8. Kiwifruit allergic children develop systemic reactions most frequently following ingestion compared to adults. Act d 1 and Act d 2 are major allergens in the pediatric age group.
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spelling pubmed-49287712016-07-12 Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition Moreno Álvarez, Ana Vila Sexto, Leticia Bardina, Luda Grishina, Galina Sampson, Hugh. A. Children (Basel) Article Kiwifruit allergy has been described mostly in the adult population, but immunoglobulin (Ig)E-mediated allergic reactions to kiwifruit appear to be occurring more frequently in children. To date, 13 allergens from kiwifruit have been identified. Our aim was to identify kiwifruit allergens in a kiwifruit allergic-pediatric population, describing clinical manifestations and patterns of recognition. Twenty-four children were included. Diagnosis of kiwifruit allergy was based on compatible clinical manifestations and demonstration of specific IgE by skin prick test (SPT) and/or serum-specific IgE determination. SDS-PAGE and immunoblotting were performed with kiwifruit extract, and proteins of interest were further analyzed by mass spectrometry/mass spectrometry. For component-resolved in vitro diagnosis, sera of kiwifruit-allergic patients were analyzed by an allergen microarray assay. Act d 1 and Act d 2 were bound by IgE from 15 of 24 children. Two children with systemic manifestations recognized a protein of 15 kDa, homologous to Act d 5. Act d 1 was the allergen with the highest frequency of recognition on microarray chip, followed by Act d 2 and Act d 8. Kiwifruit allergic children develop systemic reactions most frequently following ingestion compared to adults. Act d 1 and Act d 2 are major allergens in the pediatric age group. MDPI 2015-10-19 /pmc/articles/PMC4928771/ /pubmed/27417374 http://dx.doi.org/10.3390/children2040424 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moreno Álvarez, Ana
Vila Sexto, Leticia
Bardina, Luda
Grishina, Galina
Sampson, Hugh. A.
Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition
title Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition
title_full Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition
title_fullStr Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition
title_full_unstemmed Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition
title_short Kiwifruit Allergy in Children: Characterization of Main Allergens and Patterns of Recognition
title_sort kiwifruit allergy in children: characterization of main allergens and patterns of recognition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928771/
https://www.ncbi.nlm.nih.gov/pubmed/27417374
http://dx.doi.org/10.3390/children2040424
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