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Ultrasensitive interferometric on-chip microscopy of transparent objects

Light microscopes can detect objects through several physical processes, such as scattering, absorption, and reflection. In transparent objects, these mechanisms are often too weak, and interference effects are more suitable to observe the tiny refractive index variations that produce phase shifts....

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Detalles Bibliográficos
Autores principales: Terborg, Roland A., Pello, Josselin, Mannelli, Ilaria, Torres, Juan P., Pruneri, Valerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928994/
https://www.ncbi.nlm.nih.gov/pubmed/27386571
http://dx.doi.org/10.1126/sciadv.1600077
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author Terborg, Roland A.
Pello, Josselin
Mannelli, Ilaria
Torres, Juan P.
Pruneri, Valerio
author_facet Terborg, Roland A.
Pello, Josselin
Mannelli, Ilaria
Torres, Juan P.
Pruneri, Valerio
author_sort Terborg, Roland A.
collection PubMed
description Light microscopes can detect objects through several physical processes, such as scattering, absorption, and reflection. In transparent objects, these mechanisms are often too weak, and interference effects are more suitable to observe the tiny refractive index variations that produce phase shifts. We propose an on-chip microscope design that exploits birefringence in an unconventional geometry. It makes use of two sheared and quasi-overlapped illuminating beams experiencing relative phase shifts when going through the object, and a complementary metal-oxide-semiconductor image sensor array to record the resulting interference pattern. Unlike conventional microscopes, the beams are unfocused, leading to a very large field of view (20 mm(2)) and detection volume (more than 0.5 cm(3)), at the expense of lateral resolution. The high axial sensitivity (<1 nm) achieved using a novel phase-shifting interferometric operation makes the proposed device ideal for examining transparent substrates and reading microarrays of biomarkers. This is demonstrated by detecting nanometer-thick surface modulations on glass and single and double protein layers.
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spelling pubmed-49289942016-07-06 Ultrasensitive interferometric on-chip microscopy of transparent objects Terborg, Roland A. Pello, Josselin Mannelli, Ilaria Torres, Juan P. Pruneri, Valerio Sci Adv Research Articles Light microscopes can detect objects through several physical processes, such as scattering, absorption, and reflection. In transparent objects, these mechanisms are often too weak, and interference effects are more suitable to observe the tiny refractive index variations that produce phase shifts. We propose an on-chip microscope design that exploits birefringence in an unconventional geometry. It makes use of two sheared and quasi-overlapped illuminating beams experiencing relative phase shifts when going through the object, and a complementary metal-oxide-semiconductor image sensor array to record the resulting interference pattern. Unlike conventional microscopes, the beams are unfocused, leading to a very large field of view (20 mm(2)) and detection volume (more than 0.5 cm(3)), at the expense of lateral resolution. The high axial sensitivity (<1 nm) achieved using a novel phase-shifting interferometric operation makes the proposed device ideal for examining transparent substrates and reading microarrays of biomarkers. This is demonstrated by detecting nanometer-thick surface modulations on glass and single and double protein layers. American Association for the Advancement of Science 2016-06-10 /pmc/articles/PMC4928994/ /pubmed/27386571 http://dx.doi.org/10.1126/sciadv.1600077 Text en Copyright © 2016, The Authors http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Terborg, Roland A.
Pello, Josselin
Mannelli, Ilaria
Torres, Juan P.
Pruneri, Valerio
Ultrasensitive interferometric on-chip microscopy of transparent objects
title Ultrasensitive interferometric on-chip microscopy of transparent objects
title_full Ultrasensitive interferometric on-chip microscopy of transparent objects
title_fullStr Ultrasensitive interferometric on-chip microscopy of transparent objects
title_full_unstemmed Ultrasensitive interferometric on-chip microscopy of transparent objects
title_short Ultrasensitive interferometric on-chip microscopy of transparent objects
title_sort ultrasensitive interferometric on-chip microscopy of transparent objects
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928994/
https://www.ncbi.nlm.nih.gov/pubmed/27386571
http://dx.doi.org/10.1126/sciadv.1600077
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