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Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines

The present study investigates the anti-proliferative and apoptosis inducing mechanism of CoCl(2)·6H(2)O in PC-3 cancer cell line. Preliminary, three different forms of cobalt i.e., cobaltous (CoCl(2)·6H(2)O), macro-Co(II,III) oxide and nano-Co(II,III) oxide were screened for antiproliferative activ...

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Autores principales: Mahey, Sonia, Kumar, Rakesh, Arora, Rohit, Mahajan, Jyoti, Arora, Saroj, Bhardwaj, Renu, Thukral, Ashwani Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929113/
https://www.ncbi.nlm.nih.gov/pubmed/27386374
http://dx.doi.org/10.1186/s40064-016-2405-0
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author Mahey, Sonia
Kumar, Rakesh
Arora, Rohit
Mahajan, Jyoti
Arora, Saroj
Bhardwaj, Renu
Thukral, Ashwani Kumar
author_facet Mahey, Sonia
Kumar, Rakesh
Arora, Rohit
Mahajan, Jyoti
Arora, Saroj
Bhardwaj, Renu
Thukral, Ashwani Kumar
author_sort Mahey, Sonia
collection PubMed
description The present study investigates the anti-proliferative and apoptosis inducing mechanism of CoCl(2)·6H(2)O in PC-3 cancer cell line. Preliminary, three different forms of cobalt i.e., cobaltous (CoCl(2)·6H(2)O), macro-Co(II,III) oxide and nano-Co(II,III) oxide were screened for antiproliferative activity in PC-3 cell line. The CoCl(2)·6H(2)O being the most effective antiproliferative agent, hence it was further tested against lung (A549), prostrate (PC-3) and brain (IMR-32) cell lines. Human embryonic kidney cell line (293T) was used as a normal cell line to compare the toxicity of CoCl(2)·6H(2)O. The CoCl(2)·6H(2)O induced morphological and anatomical changes in PC-3 cancer cell which were studied using light, confocal and scanning electron microscopy. The lactate dehydrogenase was estimated which showed mild necrotic mode of cell death. The Annexin/PI staining confirmed the apoptotic mode of cell death in PC-3 cells. Further, production of reaction of reactive oxygen species and changes in mitochondrial membrane potential was also assessed spectrofluorimetrically. The cell cycle arrest was also investigated using flow cytometery. Finally, the caspase activity was estimated in CoCl(2)·6H(2)O treated PC-3 cancer cell line. Interestingly, it was found that CoCl(2)·6H(2)O induces more cell death in cancerous cells as compared to normal non-cancerous cells. These findings presented CoCl(2)·6H(2)O as potential antiproliferative agent.
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spelling pubmed-49291132016-07-06 Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines Mahey, Sonia Kumar, Rakesh Arora, Rohit Mahajan, Jyoti Arora, Saroj Bhardwaj, Renu Thukral, Ashwani Kumar Springerplus Research The present study investigates the anti-proliferative and apoptosis inducing mechanism of CoCl(2)·6H(2)O in PC-3 cancer cell line. Preliminary, three different forms of cobalt i.e., cobaltous (CoCl(2)·6H(2)O), macro-Co(II,III) oxide and nano-Co(II,III) oxide were screened for antiproliferative activity in PC-3 cell line. The CoCl(2)·6H(2)O being the most effective antiproliferative agent, hence it was further tested against lung (A549), prostrate (PC-3) and brain (IMR-32) cell lines. Human embryonic kidney cell line (293T) was used as a normal cell line to compare the toxicity of CoCl(2)·6H(2)O. The CoCl(2)·6H(2)O induced morphological and anatomical changes in PC-3 cancer cell which were studied using light, confocal and scanning electron microscopy. The lactate dehydrogenase was estimated which showed mild necrotic mode of cell death. The Annexin/PI staining confirmed the apoptotic mode of cell death in PC-3 cells. Further, production of reaction of reactive oxygen species and changes in mitochondrial membrane potential was also assessed spectrofluorimetrically. The cell cycle arrest was also investigated using flow cytometery. Finally, the caspase activity was estimated in CoCl(2)·6H(2)O treated PC-3 cancer cell line. Interestingly, it was found that CoCl(2)·6H(2)O induces more cell death in cancerous cells as compared to normal non-cancerous cells. These findings presented CoCl(2)·6H(2)O as potential antiproliferative agent. Springer International Publishing 2016-06-30 /pmc/articles/PMC4929113/ /pubmed/27386374 http://dx.doi.org/10.1186/s40064-016-2405-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Mahey, Sonia
Kumar, Rakesh
Arora, Rohit
Mahajan, Jyoti
Arora, Saroj
Bhardwaj, Renu
Thukral, Ashwani Kumar
Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
title Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
title_full Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
title_fullStr Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
title_full_unstemmed Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
title_short Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines
title_sort effect of cobalt(ii) chloride hexahydrate on some human cancer cell lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929113/
https://www.ncbi.nlm.nih.gov/pubmed/27386374
http://dx.doi.org/10.1186/s40064-016-2405-0
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