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Biophysical Characterization of Fluorotyrosine Probes Site-Specifically Incorporated into Enzymes: E. coli Ribonucleotide Reductase As an Example

[Image: see text] Fluorinated tyrosines (F(n)Y’s, n = 2 and 3) have been site-specifically incorporated into E. coli class Ia ribonucleotide reductase (RNR) using the recently evolved M. jannaschii Y-tRNA synthetase/tRNA pair. Class Ia RNRs require four redox active Y’s, a stable Y radical (Y·) in t...

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Detalles Bibliográficos
Autores principales:	Oyala, Paul H., Ravichandran, Kanchana R., Funk, Michael A., Stucky, Paul A., Stich, Troy A., Drennan, Catherine L., Britt, R. David, Stubbe, JoAnne
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2016
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929525/ https://www.ncbi.nlm.nih.gov/pubmed/27276098 http://dx.doi.org/10.1021/jacs.6b03605

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