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Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths

Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqu...

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Detalles Bibliográficos
Autores principales: Kunoh, Tatsuki, Hashimoto, Hideki, McFarlane, Ian R., Hayashi, Naoaki, Suzuki, Tomoko, Taketa, Eisuke, Tamura, Katsunori, Takano, Mikio, El-Naggar, Mohamed Y., Kunoh, Hitoshi, Takada, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929540/
https://www.ncbi.nlm.nih.gov/pubmed/27271677
http://dx.doi.org/10.3390/biology5020026
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author Kunoh, Tatsuki
Hashimoto, Hideki
McFarlane, Ian R.
Hayashi, Naoaki
Suzuki, Tomoko
Taketa, Eisuke
Tamura, Katsunori
Takano, Mikio
El-Naggar, Mohamed Y.
Kunoh, Hitoshi
Takada, Jun
author_facet Kunoh, Tatsuki
Hashimoto, Hideki
McFarlane, Ian R.
Hayashi, Naoaki
Suzuki, Tomoko
Taketa, Eisuke
Tamura, Katsunori
Takano, Mikio
El-Naggar, Mohamed Y.
Kunoh, Hitoshi
Takada, Jun
author_sort Kunoh, Tatsuki
collection PubMed
description Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 µm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths.
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spelling pubmed-49295402016-07-07 Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths Kunoh, Tatsuki Hashimoto, Hideki McFarlane, Ian R. Hayashi, Naoaki Suzuki, Tomoko Taketa, Eisuke Tamura, Katsunori Takano, Mikio El-Naggar, Mohamed Y. Kunoh, Hitoshi Takada, Jun Biology (Basel) Article Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 µm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths. MDPI 2016-06-03 /pmc/articles/PMC4929540/ /pubmed/27271677 http://dx.doi.org/10.3390/biology5020026 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kunoh, Tatsuki
Hashimoto, Hideki
McFarlane, Ian R.
Hayashi, Naoaki
Suzuki, Tomoko
Taketa, Eisuke
Tamura, Katsunori
Takano, Mikio
El-Naggar, Mohamed Y.
Kunoh, Hitoshi
Takada, Jun
Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths
title Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths
title_full Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths
title_fullStr Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths
title_full_unstemmed Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths
title_short Abiotic Deposition of Fe Complexes onto Leptothrix Sheaths
title_sort abiotic deposition of fe complexes onto leptothrix sheaths
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929540/
https://www.ncbi.nlm.nih.gov/pubmed/27271677
http://dx.doi.org/10.3390/biology5020026
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