“Cre/loxP plus BAC”: a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens

Heterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. Efforts have been made to efficiently clone gene clusters directly from genomic DNA and several approaches have been developed. Here,...

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Detalles Bibliográficos
Autores principales: Hu, Shengbiao, Liu, Zhengqiang, Zhang, Xu, Zhang, Guoyong, Xie, Yali, Ding, Xuezhi, Mo, Xiangtao, Stewart, A. Francis, Fu, Jun, Zhang, Youming, Xia, Liqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929569/
https://www.ncbi.nlm.nih.gov/pubmed/27364376
http://dx.doi.org/10.1038/srep29087

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