Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway

Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-β3 (PLC-β3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-β3 in human umbilical vein endothelial cells (HUVECs) significantly delayed proliferation, migration and...

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Autores principales: Ha, Jung Min, Baek, Seung Hoon, Kim, Young Hwan, Jin, Seo Yeon, Lee, Hye Sun, Kim, Sun Ja, Shin, Hwa Kyoung, Lee, Dong Hyung, Song, Sang Heon, Kim, Chi Dae, Bae, Sun Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
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Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929692/
https://www.ncbi.nlm.nih.gov/pubmed/27311705
http://dx.doi.org/10.1038/emm.2016.39
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author Ha, Jung Min
Baek, Seung Hoon
Kim, Young Hwan
Jin, Seo Yeon
Lee, Hye Sun
Kim, Sun Ja
Shin, Hwa Kyoung
Lee, Dong Hyung
Song, Sang Heon
Kim, Chi Dae
Bae, Sun Sik
author_facet Ha, Jung Min
Baek, Seung Hoon
Kim, Young Hwan
Jin, Seo Yeon
Lee, Hye Sun
Kim, Sun Ja
Shin, Hwa Kyoung
Lee, Dong Hyung
Song, Sang Heon
Kim, Chi Dae
Bae, Sun Sik
author_sort Ha, Jung Min
collection PubMed
description Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-β3 (PLC-β3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-β3 in human umbilical vein endothelial cells (HUVECs) significantly delayed proliferation, migration and capillary-like tube formation. In addition, mice lacking PLC-β3 showed impaired retinal angiogenesis with delayed endothelial proliferation, reduced endothelial cell activation, abnormal vessel formation and hemorrhage. Finally, tumor formation was significantly reduced in mice lacking PLC-β3 and showed irregular size and shape of blood vessels. These results suggest that regulation of endothelial function by PLC-β3 may contribute to angiogenesis.
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spelling pubmed-49296922016-07-06 Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway Ha, Jung Min Baek, Seung Hoon Kim, Young Hwan Jin, Seo Yeon Lee, Hye Sun Kim, Sun Ja Shin, Hwa Kyoung Lee, Dong Hyung Song, Sang Heon Kim, Chi Dae Bae, Sun Sik Exp Mol Med Original Article Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-β3 (PLC-β3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-β3 in human umbilical vein endothelial cells (HUVECs) significantly delayed proliferation, migration and capillary-like tube formation. In addition, mice lacking PLC-β3 showed impaired retinal angiogenesis with delayed endothelial proliferation, reduced endothelial cell activation, abnormal vessel formation and hemorrhage. Finally, tumor formation was significantly reduced in mice lacking PLC-β3 and showed irregular size and shape of blood vessels. These results suggest that regulation of endothelial function by PLC-β3 may contribute to angiogenesis. Nature Publishing Group 2016-06 2016-06-17 /pmc/articles/PMC4929692/ /pubmed/27311705 http://dx.doi.org/10.1038/emm.2016.39 Text en Copyright © 2016 KSBMB. http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Original Article
Ha, Jung Min
Baek, Seung Hoon
Kim, Young Hwan
Jin, Seo Yeon
Lee, Hye Sun
Kim, Sun Ja
Shin, Hwa Kyoung
Lee, Dong Hyung
Song, Sang Heon
Kim, Chi Dae
Bae, Sun Sik
Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
title Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
title_full Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
title_fullStr Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
title_full_unstemmed Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
title_short Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway
title_sort regulation of retinal angiogenesis by phospholipase c-β3 signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929692/
https://www.ncbi.nlm.nih.gov/pubmed/27311705
http://dx.doi.org/10.1038/emm.2016.39
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