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Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice
BACKGROUND: Most agronomical traits of crop species are complex traits controlled by multiple genes and affected by environmental factors. While considerable efforts have been made to fine-map and clone major quantitative trait loci (QTLs) for yield-related traits in rice, it is not until recently t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929766/ https://www.ncbi.nlm.nih.gov/pubmed/27363861 http://dx.doi.org/10.1186/s12863-016-0410-5 |
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author | Zhang, Hong-Wei Fan, Ye-Yang Zhu, Yu-Jun Chen, Jun-Yu Yu, Si-Bin Zhuang, Jie-Yun |
author_facet | Zhang, Hong-Wei Fan, Ye-Yang Zhu, Yu-Jun Chen, Jun-Yu Yu, Si-Bin Zhuang, Jie-Yun |
author_sort | Zhang, Hong-Wei |
collection | PubMed |
description | BACKGROUND: Most agronomical traits of crop species are complex traits controlled by multiple genes and affected by environmental factors. While considerable efforts have been made to fine-map and clone major quantitative trait loci (QTLs) for yield-related traits in rice, it is not until recently that the attention has been paid to minor QTLs. Following previous dissection of QTLs for grain weight and grain size in a 12-Mb interval on the long arm of chromosome 1 in rice, this study targeted at one putative QTL region for a more precise mapping and for analyzing the genotype-by-environment interaction of minor QTLs. RESULTS: Four BC(2)F(10) plants of the indica rice cross ZS97///ZS97//ZS97/MY46 were selected. They carried overlapped heterozygous segments that jointly covered the entire putative region for qTGW1.1 detected previously. Four sets of near isogenic lines (NILs) were developed from selfing progenies of the four plants. Each NIL set consisted of 32 ZS97 homozygous lines and 32 MY46 homozygous lines that differed in the corresponding heterozygous region. They were grown in two locations having distinct ecological conditions and measured for 1000-grain weight, grain length and grain width. Two QTLs were separated in an 835.2-kb interval flanked by DNA markers Wn28447 and RM11569. They both showed consistent effects across the two environments. The qTGW1.1a located within the 120.4-kb interval Wn28447 − RM11543 significantly affect all the three traits with the enhancing allele derived from ZS97, showing a stronger influence on grain weight than on grain length and width. The qTGW1.1b located in the 521.8-kb interval RM11554 − RM11569 significantly affect grain weight and length with the enhancing allele derived from MY46, having a stronger influence on grain length than on grain weight. Consistent performance of the two QTLs was confirmed in a validation experiment using five NIL-F(2) populations segregated for either qTGW1.1a or qTGW1.1b. CONCLUSION: Separation of closely-linked QTLs having small effects is achievable in the absence of major-QTL segregation. Minor QTLs for complex traits could act consistently in diverse environments, offering the potential of pyramiding beneficial alleles of multiple minor QTLs through marker-assisted selection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-016-0410-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4929766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49297662016-07-02 Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice Zhang, Hong-Wei Fan, Ye-Yang Zhu, Yu-Jun Chen, Jun-Yu Yu, Si-Bin Zhuang, Jie-Yun BMC Genet Research Article BACKGROUND: Most agronomical traits of crop species are complex traits controlled by multiple genes and affected by environmental factors. While considerable efforts have been made to fine-map and clone major quantitative trait loci (QTLs) for yield-related traits in rice, it is not until recently that the attention has been paid to minor QTLs. Following previous dissection of QTLs for grain weight and grain size in a 12-Mb interval on the long arm of chromosome 1 in rice, this study targeted at one putative QTL region for a more precise mapping and for analyzing the genotype-by-environment interaction of minor QTLs. RESULTS: Four BC(2)F(10) plants of the indica rice cross ZS97///ZS97//ZS97/MY46 were selected. They carried overlapped heterozygous segments that jointly covered the entire putative region for qTGW1.1 detected previously. Four sets of near isogenic lines (NILs) were developed from selfing progenies of the four plants. Each NIL set consisted of 32 ZS97 homozygous lines and 32 MY46 homozygous lines that differed in the corresponding heterozygous region. They were grown in two locations having distinct ecological conditions and measured for 1000-grain weight, grain length and grain width. Two QTLs were separated in an 835.2-kb interval flanked by DNA markers Wn28447 and RM11569. They both showed consistent effects across the two environments. The qTGW1.1a located within the 120.4-kb interval Wn28447 − RM11543 significantly affect all the three traits with the enhancing allele derived from ZS97, showing a stronger influence on grain weight than on grain length and width. The qTGW1.1b located in the 521.8-kb interval RM11554 − RM11569 significantly affect grain weight and length with the enhancing allele derived from MY46, having a stronger influence on grain length than on grain weight. Consistent performance of the two QTLs was confirmed in a validation experiment using five NIL-F(2) populations segregated for either qTGW1.1a or qTGW1.1b. CONCLUSION: Separation of closely-linked QTLs having small effects is achievable in the absence of major-QTL segregation. Minor QTLs for complex traits could act consistently in diverse environments, offering the potential of pyramiding beneficial alleles of multiple minor QTLs through marker-assisted selection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-016-0410-5) contains supplementary material, which is available to authorized users. BioMed Central 2016-06-30 /pmc/articles/PMC4929766/ /pubmed/27363861 http://dx.doi.org/10.1186/s12863-016-0410-5 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zhang, Hong-Wei Fan, Ye-Yang Zhu, Yu-Jun Chen, Jun-Yu Yu, Si-Bin Zhuang, Jie-Yun Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice |
title | Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice |
title_full | Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice |
title_fullStr | Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice |
title_full_unstemmed | Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice |
title_short | Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice |
title_sort | dissection of the qtgw1.1 region into two tightly-linked minor qtls having stable effects for grain weight in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929766/ https://www.ncbi.nlm.nih.gov/pubmed/27363861 http://dx.doi.org/10.1186/s12863-016-0410-5 |
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