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The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum
Penicillium purpurogenum is the fungus that produces an azaphilone pigment. However, details about the pigment biosynthesis pathway are unknown. The violet pigment PP-V is the one of the main pigments biosynthesized by this fungus. This pigment contains an amino group in a pyran ring as its core str...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930434/ https://www.ncbi.nlm.nih.gov/pubmed/27368914 http://dx.doi.org/10.1186/s13568-016-0215-y |
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author | Kojima, Ryo Arai, Teppei Matsufuji, Hiroshi Kasumi, Takafumi Watanabe, Taisuke Ogihara, Jun |
author_facet | Kojima, Ryo Arai, Teppei Matsufuji, Hiroshi Kasumi, Takafumi Watanabe, Taisuke Ogihara, Jun |
author_sort | Kojima, Ryo |
collection | PubMed |
description | Penicillium purpurogenum is the fungus that produces an azaphilone pigment. However, details about the pigment biosynthesis pathway are unknown. The violet pigment PP-V is the one of the main pigments biosynthesized by this fungus. This pigment contains an amino group in a pyran ring as its core structure. We focused on this pigment and examined the relationship between intracellular ammonium concentration and pigment production using glutamine as a nitrogen source. The intracellular ammonium level decreased about 1.5-fold in conditions favoring PP-V production. Moreover, P. purpurogenum was transferred to medium in which it commonly produces the related pigment PP-O after cultivating it in the presence or absence of glutamine to investigate whether this fungus biosynthesizes PP-V using surplus ammonium in cells. Only mycelia cultured in medium containing 10 mM glutamine produced the violet pigment, and simultaneously intracellular ammonium levels decreased under this condition. From comparisons of the amount of PP-V that was secreted with quantity of surplus intracellular ammonium, it is suggested that P. purpurogenum maintains ammonium homeostasis by excreting waste ammonium as PP-V. |
format | Online Article Text |
id | pubmed-4930434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-49304342016-07-06 The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum Kojima, Ryo Arai, Teppei Matsufuji, Hiroshi Kasumi, Takafumi Watanabe, Taisuke Ogihara, Jun AMB Express Original Article Penicillium purpurogenum is the fungus that produces an azaphilone pigment. However, details about the pigment biosynthesis pathway are unknown. The violet pigment PP-V is the one of the main pigments biosynthesized by this fungus. This pigment contains an amino group in a pyran ring as its core structure. We focused on this pigment and examined the relationship between intracellular ammonium concentration and pigment production using glutamine as a nitrogen source. The intracellular ammonium level decreased about 1.5-fold in conditions favoring PP-V production. Moreover, P. purpurogenum was transferred to medium in which it commonly produces the related pigment PP-O after cultivating it in the presence or absence of glutamine to investigate whether this fungus biosynthesizes PP-V using surplus ammonium in cells. Only mycelia cultured in medium containing 10 mM glutamine produced the violet pigment, and simultaneously intracellular ammonium levels decreased under this condition. From comparisons of the amount of PP-V that was secreted with quantity of surplus intracellular ammonium, it is suggested that P. purpurogenum maintains ammonium homeostasis by excreting waste ammonium as PP-V. Springer Berlin Heidelberg 2016-07-02 /pmc/articles/PMC4930434/ /pubmed/27368914 http://dx.doi.org/10.1186/s13568-016-0215-y Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Kojima, Ryo Arai, Teppei Matsufuji, Hiroshi Kasumi, Takafumi Watanabe, Taisuke Ogihara, Jun The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum |
title | The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum |
title_full | The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum |
title_fullStr | The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum |
title_full_unstemmed | The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum |
title_short | The relationship between the violet pigment PP-V production and intracellular ammonium level in Penicillium purpurogenum |
title_sort | relationship between the violet pigment pp-v production and intracellular ammonium level in penicillium purpurogenum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930434/ https://www.ncbi.nlm.nih.gov/pubmed/27368914 http://dx.doi.org/10.1186/s13568-016-0215-y |
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