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Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity

OBJECTIVE: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. MATERIALS AND MET...

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Autores principales: Sepahi, Soheila, Jafarian-Dehkordi, Abbas, Mirlohi, Maryam, Shirani, Kobra, Etebari, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930540/
https://www.ncbi.nlm.nih.gov/pubmed/27462556
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author Sepahi, Soheila
Jafarian-Dehkordi, Abbas
Mirlohi, Maryam
Shirani, Kobra
Etebari, Mahmoud
author_facet Sepahi, Soheila
Jafarian-Dehkordi, Abbas
Mirlohi, Maryam
Shirani, Kobra
Etebari, Mahmoud
author_sort Sepahi, Soheila
collection PubMed
description OBJECTIVE: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. MATERIALS AND METHODS: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. RESULTS: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. CONCLUSION: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment.
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spelling pubmed-49305402016-07-26 Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity Sepahi, Soheila Jafarian-Dehkordi, Abbas Mirlohi, Maryam Shirani, Kobra Etebari, Mahmoud Avicenna J Phytomed Original Research Article OBJECTIVE: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. MATERIALS AND METHODS: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. RESULTS: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. CONCLUSION: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment. Mashhad University of Medical Sciences 2016 /pmc/articles/PMC4930540/ /pubmed/27462556 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Sepahi, Soheila
Jafarian-Dehkordi, Abbas
Mirlohi, Maryam
Shirani, Kobra
Etebari, Mahmoud
Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
title Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
title_full Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
title_fullStr Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
title_full_unstemmed Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
title_short Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
title_sort protective role of lactobacillus plantarum a7 against irinotecan-induced genotoxicity
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930540/
https://www.ncbi.nlm.nih.gov/pubmed/27462556
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