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Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity
OBJECTIVE: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. MATERIALS AND MET...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930540/ https://www.ncbi.nlm.nih.gov/pubmed/27462556 |
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author | Sepahi, Soheila Jafarian-Dehkordi, Abbas Mirlohi, Maryam Shirani, Kobra Etebari, Mahmoud |
author_facet | Sepahi, Soheila Jafarian-Dehkordi, Abbas Mirlohi, Maryam Shirani, Kobra Etebari, Mahmoud |
author_sort | Sepahi, Soheila |
collection | PubMed |
description | OBJECTIVE: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. MATERIALS AND METHODS: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. RESULTS: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. CONCLUSION: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment. |
format | Online Article Text |
id | pubmed-4930540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-49305402016-07-26 Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity Sepahi, Soheila Jafarian-Dehkordi, Abbas Mirlohi, Maryam Shirani, Kobra Etebari, Mahmoud Avicenna J Phytomed Original Research Article OBJECTIVE: Irinotecan is a botanical derivative and an anti-cancer drug with cytotoxic and genotoxic effects. The present study evaluated the effect of Lactobacillus plantarum A7 on the genotoxic activity of irinotecan in a hepatocellular carcinoma cell line (HepG2) by comet assay. MATERIALS AND METHODS: HepG2 were incubated with irinotecan (100 µM), heat-killed cells (0.025 µg/ml) + irinotecan (100 µM), and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum A7 + irinotecan (100 µM). Phosphate buffered saline (PBS) was used as negative control. RESULTS: Irinotecan was shown to induce DNA damage in HepG2 cells. The results showed that heat-killed cells (0.025 µg/ml) and cell-free supernatants (0.5 and 1 µg/ml) of L. plantarum significantly reduce irinotecan- induced DNA damage. CONCLUSION: Our results indicate that L. plantarum A7 can decrease the genotoxic effects of irinotecan in HepG2 cells, in vitro. This finding may be supportive for the optimization of therapeutic efficacy in irinotecan treatment. Mashhad University of Medical Sciences 2016 /pmc/articles/PMC4930540/ /pubmed/27462556 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Article Sepahi, Soheila Jafarian-Dehkordi, Abbas Mirlohi, Maryam Shirani, Kobra Etebari, Mahmoud Protective role of Lactobacillus plantarum A7 against irinotecan-induced genotoxicity |
title | Protective role of Lactobacillus
plantarum A7 against irinotecan-induced genotoxicity |
title_full | Protective role of Lactobacillus
plantarum A7 against irinotecan-induced genotoxicity |
title_fullStr | Protective role of Lactobacillus
plantarum A7 against irinotecan-induced genotoxicity |
title_full_unstemmed | Protective role of Lactobacillus
plantarum A7 against irinotecan-induced genotoxicity |
title_short | Protective role of Lactobacillus
plantarum A7 against irinotecan-induced genotoxicity |
title_sort | protective role of lactobacillus
plantarum a7 against irinotecan-induced genotoxicity |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930540/ https://www.ncbi.nlm.nih.gov/pubmed/27462556 |
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