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Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis
AIMS/INTRODUCTION: The involvement of glucose‐dependent insulinotropic polypeptide (GIP) on inflammation was explored in atherosclerosis and adipose tissue. Periodontal disease is a chronic inflammatory disease, and is considered one of the diabetic complications. In the present study, to examine th...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931199/ https://www.ncbi.nlm.nih.gov/pubmed/27181102 http://dx.doi.org/10.1111/jdi.12450 |
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author | Suzuki, Yuki Nakamura, Nobuhisa Miyabe, Megumi Nishikawa, Toru Miyajima, Shin‐ichi Adachi, Kei Mizutani, Makoto Kikuchi, Takeshi Miyazawa, Ken Goto, Shigemi Tsukiyama, Katsushi Yamada, Yuichiro Ohno, Norikazu Noguchi, Toshihide Mitani, Akio Matsubara, Tatsuaki Naruse, Keiko |
author_facet | Suzuki, Yuki Nakamura, Nobuhisa Miyabe, Megumi Nishikawa, Toru Miyajima, Shin‐ichi Adachi, Kei Mizutani, Makoto Kikuchi, Takeshi Miyazawa, Ken Goto, Shigemi Tsukiyama, Katsushi Yamada, Yuichiro Ohno, Norikazu Noguchi, Toshihide Mitani, Akio Matsubara, Tatsuaki Naruse, Keiko |
author_sort | Suzuki, Yuki |
collection | PubMed |
description | AIMS/INTRODUCTION: The involvement of glucose‐dependent insulinotropic polypeptide (GIP) on inflammation was explored in atherosclerosis and adipose tissue. Periodontal disease is a chronic inflammatory disease, and is considered one of the diabetic complications. In the present study, to examine the effect of GIP on periodontitis, we induced experimental periodontitis in glucose‐dependent insulinotropic polypeptide receptor‐knockout mice (GIPRKO). We also investigated the anti‐inflammatory effect of GIP in a culture system. MATERIALS AND METHODS: Experimental periodontitis was induced by ligature wire in GIPRKO and C57BL/C mice. Two weeks after the ligature, immunohistological evaluation and inflammatory messenger ribonucleic acid expression in the gingiva was examined. To elucidate the role of GIP in inflammation, the effects of GIP on lipopolysaccharide‐induced gene expressions in THP‐1 cells were evaluated. RESULTS: Periodontitis increased inflammatory cell infiltration, macrophage accumulation and tumor necrosis factor‐α and nitric oxide synthase gene expressions in the gingiva. Periodontitis in GIPRKO showed a marked increase of inflammatory cells in the gingivomucosal tissue. Mac‐1‐positive macrophages and the inflammatory gene expressions were significantly increased in periodontitis in GIPRKO compared with C57BL/C mice periodontitis. Immunohistochemical staining confirmed that GIP receptors were expressed in residual and infiltrated Mac‐1‐positive macrophages. The in vitro study showed that GIP suppressed lipopolysaccharide‐induced tumor necrosis factor‐α and nitric oxide synthase gene expression in a dose‐dependent manner. Furthermore, the inhibitory effect of GIP on lipopolysaccharide‐induced inflammatory gene expressions was at least partially through cyclic adenosine monophosphate/protein kinase A pathway. CONCLUSIONS: These results suggest the beneficial effects of GIP on periodontal disease. In diabetic patients, GIP is expected to have a direct anti‐inflammatory effect on periodontitis in addition to its glucose‐lowering effect. |
format | Online Article Text |
id | pubmed-4931199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-49311992016-07-06 Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis Suzuki, Yuki Nakamura, Nobuhisa Miyabe, Megumi Nishikawa, Toru Miyajima, Shin‐ichi Adachi, Kei Mizutani, Makoto Kikuchi, Takeshi Miyazawa, Ken Goto, Shigemi Tsukiyama, Katsushi Yamada, Yuichiro Ohno, Norikazu Noguchi, Toshihide Mitani, Akio Matsubara, Tatsuaki Naruse, Keiko J Diabetes Investig Articles AIMS/INTRODUCTION: The involvement of glucose‐dependent insulinotropic polypeptide (GIP) on inflammation was explored in atherosclerosis and adipose tissue. Periodontal disease is a chronic inflammatory disease, and is considered one of the diabetic complications. In the present study, to examine the effect of GIP on periodontitis, we induced experimental periodontitis in glucose‐dependent insulinotropic polypeptide receptor‐knockout mice (GIPRKO). We also investigated the anti‐inflammatory effect of GIP in a culture system. MATERIALS AND METHODS: Experimental periodontitis was induced by ligature wire in GIPRKO and C57BL/C mice. Two weeks after the ligature, immunohistological evaluation and inflammatory messenger ribonucleic acid expression in the gingiva was examined. To elucidate the role of GIP in inflammation, the effects of GIP on lipopolysaccharide‐induced gene expressions in THP‐1 cells were evaluated. RESULTS: Periodontitis increased inflammatory cell infiltration, macrophage accumulation and tumor necrosis factor‐α and nitric oxide synthase gene expressions in the gingiva. Periodontitis in GIPRKO showed a marked increase of inflammatory cells in the gingivomucosal tissue. Mac‐1‐positive macrophages and the inflammatory gene expressions were significantly increased in periodontitis in GIPRKO compared with C57BL/C mice periodontitis. Immunohistochemical staining confirmed that GIP receptors were expressed in residual and infiltrated Mac‐1‐positive macrophages. The in vitro study showed that GIP suppressed lipopolysaccharide‐induced tumor necrosis factor‐α and nitric oxide synthase gene expression in a dose‐dependent manner. Furthermore, the inhibitory effect of GIP on lipopolysaccharide‐induced inflammatory gene expressions was at least partially through cyclic adenosine monophosphate/protein kinase A pathway. CONCLUSIONS: These results suggest the beneficial effects of GIP on periodontal disease. In diabetic patients, GIP is expected to have a direct anti‐inflammatory effect on periodontitis in addition to its glucose‐lowering effect. John Wiley and Sons Inc. 2016-01-07 2016-07 /pmc/articles/PMC4931199/ /pubmed/27181102 http://dx.doi.org/10.1111/jdi.12450 Text en © 2015 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Articles Suzuki, Yuki Nakamura, Nobuhisa Miyabe, Megumi Nishikawa, Toru Miyajima, Shin‐ichi Adachi, Kei Mizutani, Makoto Kikuchi, Takeshi Miyazawa, Ken Goto, Shigemi Tsukiyama, Katsushi Yamada, Yuichiro Ohno, Norikazu Noguchi, Toshihide Mitani, Akio Matsubara, Tatsuaki Naruse, Keiko Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
title | Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
title_full | Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
title_fullStr | Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
title_full_unstemmed | Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
title_short | Anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
title_sort | anti‐inflammatory role of glucose‐dependent insulinotropic polypeptide in periodontitis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931199/ https://www.ncbi.nlm.nih.gov/pubmed/27181102 http://dx.doi.org/10.1111/jdi.12450 |
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