A Flow SPR Immunosensor Based on a Sandwich Direct Method

In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods di...

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Detalles Bibliográficos
Autores principales: Tomassetti, Mauro, Conta, Giorgia, Campanella, Luigi, Favero, Gabriele, Sanzò, Gabriella, Mazzei, Franco, Antiochia, Riccarda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931482/
https://www.ncbi.nlm.nih.gov/pubmed/27187486
http://dx.doi.org/10.3390/bios6020022
Descripción
Sumario:In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(−3) and 10(−1) M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes.

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