A Flow SPR Immunosensor Based on a Sandwich Direct Method

In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods di...

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Detalles Bibliográficos
Autores principales: Tomassetti, Mauro, Conta, Giorgia, Campanella, Luigi, Favero, Gabriele, Sanzò, Gabriella, Mazzei, Franco, Antiochia, Riccarda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931482/
https://www.ncbi.nlm.nih.gov/pubmed/27187486
http://dx.doi.org/10.3390/bios6020022
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author Tomassetti, Mauro
Conta, Giorgia
Campanella, Luigi
Favero, Gabriele
Sanzò, Gabriella
Mazzei, Franco
Antiochia, Riccarda
author_facet Tomassetti, Mauro
Conta, Giorgia
Campanella, Luigi
Favero, Gabriele
Sanzò, Gabriella
Mazzei, Franco
Antiochia, Riccarda
author_sort Tomassetti, Mauro
collection PubMed
description In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(−3) and 10(−1) M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes.
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spelling pubmed-49314822016-07-08 A Flow SPR Immunosensor Based on a Sandwich Direct Method Tomassetti, Mauro Conta, Giorgia Campanella, Luigi Favero, Gabriele Sanzò, Gabriella Mazzei, Franco Antiochia, Riccarda Biosensors (Basel) Article In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(−3) and 10(−1) M, a measurement time of ≤20 min and a high selectivity both towards β-lactam antibiotics and antibiotics of different classes. MDPI 2016-05-13 /pmc/articles/PMC4931482/ /pubmed/27187486 http://dx.doi.org/10.3390/bios6020022 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomassetti, Mauro
Conta, Giorgia
Campanella, Luigi
Favero, Gabriele
Sanzò, Gabriella
Mazzei, Franco
Antiochia, Riccarda
A Flow SPR Immunosensor Based on a Sandwich Direct Method
title A Flow SPR Immunosensor Based on a Sandwich Direct Method
title_full A Flow SPR Immunosensor Based on a Sandwich Direct Method
title_fullStr A Flow SPR Immunosensor Based on a Sandwich Direct Method
title_full_unstemmed A Flow SPR Immunosensor Based on a Sandwich Direct Method
title_short A Flow SPR Immunosensor Based on a Sandwich Direct Method
title_sort flow spr immunosensor based on a sandwich direct method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931482/
https://www.ncbi.nlm.nih.gov/pubmed/27187486
http://dx.doi.org/10.3390/bios6020022
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