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Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors

Acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. However, a newly identified isolate of this species from a frost-plant-tissue sample, namely, A. johnsonii MB44, showed significant nematicidal activity against the model...

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Autores principales: Tian, Shijing, Ali, Muhammad, Xie, Li, Li, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932006/
https://www.ncbi.nlm.nih.gov/pubmed/27429894
http://dx.doi.org/10.1186/s40064-016-2668-5
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author Tian, Shijing
Ali, Muhammad
Xie, Li
Li, Lin
author_facet Tian, Shijing
Ali, Muhammad
Xie, Li
Li, Lin
author_sort Tian, Shijing
collection PubMed
description Acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. However, a newly identified isolate of this species from a frost-plant-tissue sample, namely, A. johnsonii MB44, showed significant nematicidal activity against the model organism Caenorhabditis elegans. To expand our understanding of this bacterial species, we generated a draft genome sequence of MB44 and analyzed its genomic features related to nematicidal attributes. The 3.36 Mb long genome contains 3636 predicted protein-coding genes and 95 RNA genes (including 14 rRNA genes), with a G + C content of 41.37 %. Genomic analysis of the prediction of nematicidal proteins using the software MP3 revealed a total of 108 potential virulence proteins. Some of these proteins were homologous to the known virulent proteins identified from Acinetobacter baumannii, a pathogenic species of the genus Acinetobacter. These virulent proteins included the outer membrane protein A, the phospholipase D, and penicillin-binding protein 7/8. Moreover, one siderophore biosynthesis gene cluster and one capsular polysaccharide gene cluster, which were predicted to be important virulence factors for C. elegans, were identified in the MB44 genome. The current study demonstrated that A. johnsonii MB44, with its nematicidal activity, could be an opportunistic pathogen to animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-2668-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-49320062016-07-16 Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors Tian, Shijing Ali, Muhammad Xie, Li Li, Lin Springerplus Research Acinetobacter johnsonii is generally recognized as a nonpathogenic bacterium although it is often found in hospital environments. However, a newly identified isolate of this species from a frost-plant-tissue sample, namely, A. johnsonii MB44, showed significant nematicidal activity against the model organism Caenorhabditis elegans. To expand our understanding of this bacterial species, we generated a draft genome sequence of MB44 and analyzed its genomic features related to nematicidal attributes. The 3.36 Mb long genome contains 3636 predicted protein-coding genes and 95 RNA genes (including 14 rRNA genes), with a G + C content of 41.37 %. Genomic analysis of the prediction of nematicidal proteins using the software MP3 revealed a total of 108 potential virulence proteins. Some of these proteins were homologous to the known virulent proteins identified from Acinetobacter baumannii, a pathogenic species of the genus Acinetobacter. These virulent proteins included the outer membrane protein A, the phospholipase D, and penicillin-binding protein 7/8. Moreover, one siderophore biosynthesis gene cluster and one capsular polysaccharide gene cluster, which were predicted to be important virulence factors for C. elegans, were identified in the MB44 genome. The current study demonstrated that A. johnsonii MB44, with its nematicidal activity, could be an opportunistic pathogen to animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-2668-5) contains supplementary material, which is available to authorized users. Springer International Publishing 2016-07-04 /pmc/articles/PMC4932006/ /pubmed/27429894 http://dx.doi.org/10.1186/s40064-016-2668-5 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Tian, Shijing
Ali, Muhammad
Xie, Li
Li, Lin
Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors
title Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors
title_full Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors
title_fullStr Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors
title_full_unstemmed Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors
title_short Genome-sequence analysis of Acinetobacter johnsonii MB44 reveals potential nematode-virulent factors
title_sort genome-sequence analysis of acinetobacter johnsonii mb44 reveals potential nematode-virulent factors
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932006/
https://www.ncbi.nlm.nih.gov/pubmed/27429894
http://dx.doi.org/10.1186/s40064-016-2668-5
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