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Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis

Tdrd12 is one of tudor domain containing (Tdrd) family members. However, the expression pattern of Tdrd12 has not been well studied. To compare the expression levels of Tdrd12 in various tissues, real time-polymerase chain reaction was performed using total RNAs from liver, small intestine, heart, b...

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Autores principales: Kim, Min, Ki, Byeong Seong, Hong, Kwonho, Park, Se-pill, Ko, Jung-Jae, Choi, Youngsok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932588/
https://www.ncbi.nlm.nih.gov/pubmed/26954166
http://dx.doi.org/10.5713/ajas.15.0436
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author Kim, Min
Ki, Byeong Seong
Hong, Kwonho
Park, Se-pill
Ko, Jung-Jae
Choi, Youngsok
author_facet Kim, Min
Ki, Byeong Seong
Hong, Kwonho
Park, Se-pill
Ko, Jung-Jae
Choi, Youngsok
author_sort Kim, Min
collection PubMed
description Tdrd12 is one of tudor domain containing (Tdrd) family members. However, the expression pattern of Tdrd12 has not been well studied. To compare the expression levels of Tdrd12 in various tissues, real time-polymerase chain reaction was performed using total RNAs from liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Tdrd12 mRNA was highly expressed in testis. Antibody against mouse TDRD12 were generated using amino acid residues SQRPNEKPLRLTEKKDC of TDRD12 to investigate TDRD12 localization in testis. Immunostaining assay shows that TDRD12 is mainly localized at the spermatid in the seminiferous tubules of adult testes. During postnatal development, TDRD12 is differentially expressed. TDRD12 was detected in early spermatocytes at 2 weeks and TDRD12 was localized at acrosome of the round spermatids. TDRD12 expression was not co-localized with TDRD1 which is an important component of piRNA pathway in germ cells. Our results indicate that TDRD12 may play an important role in spermatids and function as a regulator of spermatogenesis in dependent of TDRD1.
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spelling pubmed-49325882016-07-07 Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis Kim, Min Ki, Byeong Seong Hong, Kwonho Park, Se-pill Ko, Jung-Jae Choi, Youngsok Asian-Australas J Anim Sci Article Tdrd12 is one of tudor domain containing (Tdrd) family members. However, the expression pattern of Tdrd12 has not been well studied. To compare the expression levels of Tdrd12 in various tissues, real time-polymerase chain reaction was performed using total RNAs from liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Tdrd12 mRNA was highly expressed in testis. Antibody against mouse TDRD12 were generated using amino acid residues SQRPNEKPLRLTEKKDC of TDRD12 to investigate TDRD12 localization in testis. Immunostaining assay shows that TDRD12 is mainly localized at the spermatid in the seminiferous tubules of adult testes. During postnatal development, TDRD12 is differentially expressed. TDRD12 was detected in early spermatocytes at 2 weeks and TDRD12 was localized at acrosome of the round spermatids. TDRD12 expression was not co-localized with TDRD1 which is an important component of piRNA pathway in germ cells. Our results indicate that TDRD12 may play an important role in spermatids and function as a regulator of spermatogenesis in dependent of TDRD1. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2016-07 2015-09-30 /pmc/articles/PMC4932588/ /pubmed/26954166 http://dx.doi.org/10.5713/ajas.15.0436 Text en Copyright © 2016 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kim, Min
Ki, Byeong Seong
Hong, Kwonho
Park, Se-pill
Ko, Jung-Jae
Choi, Youngsok
Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis
title Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis
title_full Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis
title_fullStr Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis
title_full_unstemmed Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis
title_short Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis
title_sort tudor domain containing protein tdrd12 expresses at the acrosome of spermatids in mouse testis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4932588/
https://www.ncbi.nlm.nih.gov/pubmed/26954166
http://dx.doi.org/10.5713/ajas.15.0436
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