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Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo
We describe an all-optical strategy for simultaneously manipulating and recording the activity of multiple neurons with cellular resolution in vivo. Concurrent two-photon optogenetic activation and calcium imaging is enabled by coexpression of a red-shifted opsin and a genetically encoded calcium in...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933203/ https://www.ncbi.nlm.nih.gov/pubmed/25532138 http://dx.doi.org/10.1038/nmeth.3217 |