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Expression of the Endocannabinoid Receptors in Human Fascial Tissue
Cannabinoid receptors have been localized in the central and peripheral nervous system as well as on cells of the immune system, but recent studies on animal tissue gave evidence for the presence of cannabinoid receptors in different types of tissues. Their presence was supposed also in myofascial t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications, Pavia, Italy
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933831/ https://www.ncbi.nlm.nih.gov/pubmed/27349320 http://dx.doi.org/10.4081/ejh.2016.2643 |
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author | Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. Caro, R. De Stecco, C. |
author_facet | Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. Caro, R. De Stecco, C. |
author_sort | Fede, C. |
collection | PubMed |
description | Cannabinoid receptors have been localized in the central and peripheral nervous system as well as on cells of the immune system, but recent studies on animal tissue gave evidence for the presence of cannabinoid receptors in different types of tissues. Their presence was supposed also in myofascial tissue, suggesting that the endocannabinoid system may help resolve myofascial trigger points and relieve symptoms of fibromyalgia. However, until now the expression of CB1 (cannabinoid receptor 1) and CB2 (cannabinoid receptor 2) in fasciae has not yet been established. Small samples of fascia were collected from volunteers patients during orthopedic surgery. For each sample were done a cell isolation, immunohistochemical investigation (CB1 and CB2 antibodies) and real time RT-PCR to detect the expression of CB1 and CB2. Both cannabinoid receptors are expressed in human fascia and in human fascial fibroblasts culture cells, although to a lesser extent than the control gene. We can assume that the expression of mRNA and protein of CB1 and CB2 receptors in fascial tissue are concentrated into the fibroblasts. This is the first demonstration that the fibroblasts of the muscular fasciae express CB1 and CB2. The presence of these receptors could help to provide a description of cannabinoid receptors distribution and to better explain the role of fasciae as pain generator and the efficacy of some fascial treatments. Indeed the endocannabinoid receptors of fascial fibroblasts can contribute to modulate the fascial fibrosis and inflammation. |
format | Online Article Text |
id | pubmed-4933831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PAGEPress Publications, Pavia, Italy |
record_format | MEDLINE/PubMed |
spelling | pubmed-49338312016-07-18 Expression of the Endocannabinoid Receptors in Human Fascial Tissue Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. Caro, R. De Stecco, C. Eur J Histochem Original Paper Cannabinoid receptors have been localized in the central and peripheral nervous system as well as on cells of the immune system, but recent studies on animal tissue gave evidence for the presence of cannabinoid receptors in different types of tissues. Their presence was supposed also in myofascial tissue, suggesting that the endocannabinoid system may help resolve myofascial trigger points and relieve symptoms of fibromyalgia. However, until now the expression of CB1 (cannabinoid receptor 1) and CB2 (cannabinoid receptor 2) in fasciae has not yet been established. Small samples of fascia were collected from volunteers patients during orthopedic surgery. For each sample were done a cell isolation, immunohistochemical investigation (CB1 and CB2 antibodies) and real time RT-PCR to detect the expression of CB1 and CB2. Both cannabinoid receptors are expressed in human fascia and in human fascial fibroblasts culture cells, although to a lesser extent than the control gene. We can assume that the expression of mRNA and protein of CB1 and CB2 receptors in fascial tissue are concentrated into the fibroblasts. This is the first demonstration that the fibroblasts of the muscular fasciae express CB1 and CB2. The presence of these receptors could help to provide a description of cannabinoid receptors distribution and to better explain the role of fasciae as pain generator and the efficacy of some fascial treatments. Indeed the endocannabinoid receptors of fascial fibroblasts can contribute to modulate the fascial fibrosis and inflammation. PAGEPress Publications, Pavia, Italy 2016-06-28 /pmc/articles/PMC4933831/ /pubmed/27349320 http://dx.doi.org/10.4081/ejh.2016.2643 Text en ©Copyright C. Fede et al. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Fede, C. Albertin, G. Petrelli, L. Sfriso, M.M. Biz, C. Caro, R. De Stecco, C. Expression of the Endocannabinoid Receptors in Human Fascial Tissue |
title | Expression of the Endocannabinoid Receptors in Human Fascial Tissue |
title_full | Expression of the Endocannabinoid Receptors in Human Fascial Tissue |
title_fullStr | Expression of the Endocannabinoid Receptors in Human Fascial Tissue |
title_full_unstemmed | Expression of the Endocannabinoid Receptors in Human Fascial Tissue |
title_short | Expression of the Endocannabinoid Receptors in Human Fascial Tissue |
title_sort | expression of the endocannabinoid receptors in human fascial tissue |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933831/ https://www.ncbi.nlm.nih.gov/pubmed/27349320 http://dx.doi.org/10.4081/ejh.2016.2643 |
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