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Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology

A straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of t...

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Autores principales: Hynninen, Ville, Vuori, Leena, Hannula, Markku, Tapio, Kosti, Lahtonen, Kimmo, Isoniemi, Tommi, Lehtonen, Elina, Hirsimäki, Mika, Toppari, J. Jussi, Valden, Mika, Hytönen, Vesa P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933944/
https://www.ncbi.nlm.nih.gov/pubmed/27381834
http://dx.doi.org/10.1038/srep29324
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author Hynninen, Ville
Vuori, Leena
Hannula, Markku
Tapio, Kosti
Lahtonen, Kimmo
Isoniemi, Tommi
Lehtonen, Elina
Hirsimäki, Mika
Toppari, J. Jussi
Valden, Mika
Hytönen, Vesa P.
author_facet Hynninen, Ville
Vuori, Leena
Hannula, Markku
Tapio, Kosti
Lahtonen, Kimmo
Isoniemi, Tommi
Lehtonen, Elina
Hirsimäki, Mika
Toppari, J. Jussi
Valden, Mika
Hytönen, Vesa P.
author_sort Hynninen, Ville
collection PubMed
description A straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of the modified surface were achieved. According to photoelectron spectroscopy analyses, the silane-PEGs formed less than 10 Å thick overlayers with close to 90% surface coverage and reproducible chemical compositions. Consequently, the surfaces also became more hydrophilic, and the observed non-specific biofouling of proteins was reduced by approximately 70%. In addition, the attachment of E. coli was reduced by more than 65%. Moreover, the potential of the overlayer to be further modified was demonstrated by successfully coupling biotinylated alkaline phosphatase (bAP) to a silane-PEG-biotin overlayer via avidin-biotin bridges. The activity of the immobilized enzyme was shown to be well preserved without compromising the achieved antifouling properties. Overall, the simple solution-based approach enables the tailoring of SS to enhance its activity for biomedical and biotechnological applications.
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spelling pubmed-49339442016-07-08 Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology Hynninen, Ville Vuori, Leena Hannula, Markku Tapio, Kosti Lahtonen, Kimmo Isoniemi, Tommi Lehtonen, Elina Hirsimäki, Mika Toppari, J. Jussi Valden, Mika Hytönen, Vesa P. Sci Rep Article A straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of the modified surface were achieved. According to photoelectron spectroscopy analyses, the silane-PEGs formed less than 10 Å thick overlayers with close to 90% surface coverage and reproducible chemical compositions. Consequently, the surfaces also became more hydrophilic, and the observed non-specific biofouling of proteins was reduced by approximately 70%. In addition, the attachment of E. coli was reduced by more than 65%. Moreover, the potential of the overlayer to be further modified was demonstrated by successfully coupling biotinylated alkaline phosphatase (bAP) to a silane-PEG-biotin overlayer via avidin-biotin bridges. The activity of the immobilized enzyme was shown to be well preserved without compromising the achieved antifouling properties. Overall, the simple solution-based approach enables the tailoring of SS to enhance its activity for biomedical and biotechnological applications. Nature Publishing Group 2016-07-06 /pmc/articles/PMC4933944/ /pubmed/27381834 http://dx.doi.org/10.1038/srep29324 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hynninen, Ville
Vuori, Leena
Hannula, Markku
Tapio, Kosti
Lahtonen, Kimmo
Isoniemi, Tommi
Lehtonen, Elina
Hirsimäki, Mika
Toppari, J. Jussi
Valden, Mika
Hytönen, Vesa P.
Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
title Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
title_full Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
title_fullStr Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
title_full_unstemmed Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
title_short Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
title_sort improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933944/
https://www.ncbi.nlm.nih.gov/pubmed/27381834
http://dx.doi.org/10.1038/srep29324
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