Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR

BACKGROUND: The success of the CRISPR/Cas9 genome editing technique depends on the choice of the guide RNA sequence, which is facilitated by various websites. Despite the importance and popularity of these algorithms, it is unclear to which extent their predictions are in agreement with actual measu...

Descripción completa

Detalles Bibliográficos
Autores principales: Haeussler, Maximilian, Schönig, Kai, Eckert, Hélène, Eschstruth, Alexis, Mianné, Joffrey, Renaud, Jean-Baptiste, Schneider-Maunoury, Sylvie, Shkumatava, Alena, Teboul, Lydia, Kent, Jim, Joly, Jean-Stephane, Concordet, Jean-Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934014/
https://www.ncbi.nlm.nih.gov/pubmed/27380939
http://dx.doi.org/10.1186/s13059-016-1012-2

Ejemplares similares

Ejemplares similares