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Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets

We developed a new method for detecting S1 nuclease and hydroxyl radicals based on the use of water-soluble conjugated poly[9,9-bis(6,6-(N,N,N-trimethylammonium)-fluorene)-2,7-ylenevinylene-co-alt-2,5-dicyano-1,4-phenylene)] (PFVCN) and tungsten disulfide (WS(2)) nanosheets(.) Cationic PFVCN is used...

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Autores principales: Li, Junting, Zhao, Qi, Tang, Yanli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934291/
https://www.ncbi.nlm.nih.gov/pubmed/27304956
http://dx.doi.org/10.3390/s16060865
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author Li, Junting
Zhao, Qi
Tang, Yanli
author_facet Li, Junting
Zhao, Qi
Tang, Yanli
author_sort Li, Junting
collection PubMed
description We developed a new method for detecting S1 nuclease and hydroxyl radicals based on the use of water-soluble conjugated poly[9,9-bis(6,6-(N,N,N-trimethylammonium)-fluorene)-2,7-ylenevinylene-co-alt-2,5-dicyano-1,4-phenylene)] (PFVCN) and tungsten disulfide (WS(2)) nanosheets(.) Cationic PFVCN is used as a signal reporter, and single-layer WS(2) is used as a quencher with a negatively charged surface. The ssDNA forms complexes with PFVCN due to much stronger electrostatic interactions between cationic PFVCN and anionic ssDNA, whereas PFVCN emits yellow fluorescence. When ssDNA is hydrolyzed by S1 nuclease or hydroxyl radicals into small fragments, the interactions between the fragmented DNA and PFVCN become weaker, resulting in PFVCN being adsorbed on the surface of WS(2) and the fluorescence being quenched through fluorescence resonance energy transfer. The new method based on PFVCN and WS(2) can sense S1 nuclease with a low detection limit of 5 × 10(−6) U/mL. Additionally, this method is cost-effective by using affordable WS(2) as an energy acceptor without the need for dye-labeled ssDNA. Furthermore, the method provides a new platform for the nuclease assay and reactive oxygen species, and provides promising applications for drug screening.
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spelling pubmed-49342912016-07-06 Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets Li, Junting Zhao, Qi Tang, Yanli Sensors (Basel) Article We developed a new method for detecting S1 nuclease and hydroxyl radicals based on the use of water-soluble conjugated poly[9,9-bis(6,6-(N,N,N-trimethylammonium)-fluorene)-2,7-ylenevinylene-co-alt-2,5-dicyano-1,4-phenylene)] (PFVCN) and tungsten disulfide (WS(2)) nanosheets(.) Cationic PFVCN is used as a signal reporter, and single-layer WS(2) is used as a quencher with a negatively charged surface. The ssDNA forms complexes with PFVCN due to much stronger electrostatic interactions between cationic PFVCN and anionic ssDNA, whereas PFVCN emits yellow fluorescence. When ssDNA is hydrolyzed by S1 nuclease or hydroxyl radicals into small fragments, the interactions between the fragmented DNA and PFVCN become weaker, resulting in PFVCN being adsorbed on the surface of WS(2) and the fluorescence being quenched through fluorescence resonance energy transfer. The new method based on PFVCN and WS(2) can sense S1 nuclease with a low detection limit of 5 × 10(−6) U/mL. Additionally, this method is cost-effective by using affordable WS(2) as an energy acceptor without the need for dye-labeled ssDNA. Furthermore, the method provides a new platform for the nuclease assay and reactive oxygen species, and provides promising applications for drug screening. MDPI 2016-06-13 /pmc/articles/PMC4934291/ /pubmed/27304956 http://dx.doi.org/10.3390/s16060865 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Junting
Zhao, Qi
Tang, Yanli
Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets
title Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets
title_full Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets
title_fullStr Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets
title_full_unstemmed Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets
title_short Label-Free Fluorescence Assay of S1 Nuclease and Hydroxyl Radicals Based on Water-Soluble Conjugated Polymers and WS(2) Nanosheets
title_sort label-free fluorescence assay of s1 nuclease and hydroxyl radicals based on water-soluble conjugated polymers and ws(2) nanosheets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934291/
https://www.ncbi.nlm.nih.gov/pubmed/27304956
http://dx.doi.org/10.3390/s16060865
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AT tangyanli labelfreefluorescenceassayofs1nucleaseandhydroxylradicalsbasedonwatersolubleconjugatedpolymersandws2nanosheets