Quantification of designer nuclease induced mutation rates: a direct comparison of different methods

Designer nucleases are broadly applied to induce site-specific DNA double-strand breaks (DSB) in genomic DNA. These are repaired by nonhomologous end joining leading to insertions or deletions (in/dels) at the respective DNA-locus. To detect in/del mutations, the heteroduplex based T7-endonuclease I...

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Detalles Bibliográficos
Autores principales: Ehrke-Schulz, Eric, Bergmann, Thorsten, Schiwon, Maren, Doerner, Johannes, Saydaminova, Kamola, Lieber, Andre, Ehrhardt, Anja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934480/
https://www.ncbi.nlm.nih.gov/pubmed/27419195
http://dx.doi.org/10.1038/mtm.2016.47

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934480/
https://www.ncbi.nlm.nih.gov/pubmed/27419195
http://dx.doi.org/10.1038/mtm.2016.47

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