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Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats

Bubali Cornu (water buffalo horn, WBH) has been used for thousands of years in traditional Chinese medicine (TCM) as an effective treatment for heat. In the present study, we have carried out a metabolomics profiling study on plasma and urine samples to explore potential biomarkers and determine how...

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Autores principales: Liu, Rui, Huang, Qiong, Shan, Jinjun, Duan, Jin-ao, Zhu, Zhenhua, Liu, Pei, Bian, Yong, Shang, Er-xin, Qian, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934856/
https://www.ncbi.nlm.nih.gov/pubmed/27384078
http://dx.doi.org/10.1371/journal.pone.0158478
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author Liu, Rui
Huang, Qiong
Shan, Jinjun
Duan, Jin-ao
Zhu, Zhenhua
Liu, Pei
Bian, Yong
Shang, Er-xin
Qian, Dawei
author_facet Liu, Rui
Huang, Qiong
Shan, Jinjun
Duan, Jin-ao
Zhu, Zhenhua
Liu, Pei
Bian, Yong
Shang, Er-xin
Qian, Dawei
author_sort Liu, Rui
collection PubMed
description Bubali Cornu (water buffalo horn, WBH) has been used for thousands of years in traditional Chinese medicine (TCM) as an effective treatment for heat. In the present study, we have carried out a metabolomics profiling study on plasma and urine samples to explore potential biomarkers and determine how WBH exerts its antipyretic effects in yeast-induced pyrexia at a metabolomic level. Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS), together with multivariate statistical analysis, was used to detect and identify potential biomarkers associated with pyrexia and with WBH treatment. In total, sixteen endogenous metabolites were identified in plasma samples and twenty-one metabolites were detected in urine samples. The biomarkers identified in this study, using metabolic pathway analysis (MetPA), are involved in glycerophospholipid, arachidonic acid, amino acid, sphingolipid, and purine metabolism, all of which are disturbed in rats with pyrexia. As a result, WBH affect arachidonic acid metabolism and oxidative stress in yeast-induced pyrexia rats chiefly. The present study determines the important substances underlying the antipyretic efficacy of WBH at a metabolic level. It might pave the way for further investigations into the mechanisms of action of other animal horn-derived traditional Chinese medicines (TCMs).
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spelling pubmed-49348562016-07-18 Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats Liu, Rui Huang, Qiong Shan, Jinjun Duan, Jin-ao Zhu, Zhenhua Liu, Pei Bian, Yong Shang, Er-xin Qian, Dawei PLoS One Research Article Bubali Cornu (water buffalo horn, WBH) has been used for thousands of years in traditional Chinese medicine (TCM) as an effective treatment for heat. In the present study, we have carried out a metabolomics profiling study on plasma and urine samples to explore potential biomarkers and determine how WBH exerts its antipyretic effects in yeast-induced pyrexia at a metabolomic level. Ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS), together with multivariate statistical analysis, was used to detect and identify potential biomarkers associated with pyrexia and with WBH treatment. In total, sixteen endogenous metabolites were identified in plasma samples and twenty-one metabolites were detected in urine samples. The biomarkers identified in this study, using metabolic pathway analysis (MetPA), are involved in glycerophospholipid, arachidonic acid, amino acid, sphingolipid, and purine metabolism, all of which are disturbed in rats with pyrexia. As a result, WBH affect arachidonic acid metabolism and oxidative stress in yeast-induced pyrexia rats chiefly. The present study determines the important substances underlying the antipyretic efficacy of WBH at a metabolic level. It might pave the way for further investigations into the mechanisms of action of other animal horn-derived traditional Chinese medicines (TCMs). Public Library of Science 2016-07-06 /pmc/articles/PMC4934856/ /pubmed/27384078 http://dx.doi.org/10.1371/journal.pone.0158478 Text en © 2016 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liu, Rui
Huang, Qiong
Shan, Jinjun
Duan, Jin-ao
Zhu, Zhenhua
Liu, Pei
Bian, Yong
Shang, Er-xin
Qian, Dawei
Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats
title Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats
title_full Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats
title_fullStr Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats
title_full_unstemmed Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats
title_short Metabolomics of the Antipyretic Effects of Bubali Cornu (Water Buffalo Horn) in Rats
title_sort metabolomics of the antipyretic effects of bubali cornu (water buffalo horn) in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934856/
https://www.ncbi.nlm.nih.gov/pubmed/27384078
http://dx.doi.org/10.1371/journal.pone.0158478
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