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Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback

Perchlorate is a common aquatic contaminant that has long been known to affect thyroid function in vertebrates, including humans. More recently perchlorate has been shown to affect primordial sexual differentiation in the aquatic model fishes zebrafish and threespine stickleback, but the mechanism h...

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Autores principales: Petersen, Ann M., Earp, Nathanial C., Redmond, Mandy E., Postlethwait, John H., von Hippel, Frank A., Buck, C. Loren, Cresko, William A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934864/
https://www.ncbi.nlm.nih.gov/pubmed/27383240
http://dx.doi.org/10.1371/journal.pone.0157792
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author Petersen, Ann M.
Earp, Nathanial C.
Redmond, Mandy E.
Postlethwait, John H.
von Hippel, Frank A.
Buck, C. Loren
Cresko, William A.
author_facet Petersen, Ann M.
Earp, Nathanial C.
Redmond, Mandy E.
Postlethwait, John H.
von Hippel, Frank A.
Buck, C. Loren
Cresko, William A.
author_sort Petersen, Ann M.
collection PubMed
description Perchlorate is a common aquatic contaminant that has long been known to affect thyroid function in vertebrates, including humans. More recently perchlorate has been shown to affect primordial sexual differentiation in the aquatic model fishes zebrafish and threespine stickleback, but the mechanism has been unclear. Stickleback exposed to perchlorate from fertilization have increased androgen levels in the embryo and disrupted reproductive morphologies as adults, suggesting that perchlorate could disrupt the earliest stages of primordial sexual differentiation when primordial germ cells (PGCs) begin to form the gonad. Female stickleback have three to four times the number of PGCs as males during the first weeks of development. We hypothesized that perchlorate exposure affects primordial sexual differentiation by reducing the number of germ cells in the gonad during an important window of stickleback sex determination at 14–18 days post fertilization (dpf). We tested this hypothesis by quantifying the number of PGCs at 16 dpf in control and 100 mg/L perchlorate-treated male and female stickleback. Perchlorate exposure from the time of fertilization resulted in significantly reduced PGC number only in genotypic females, suggesting that the masculinizing effects of perchlorate observed in adult stickleback may result from early changes to the number of PGCs at a time critical for sex determination. To our knowledge, this is the first evidence of a connection between an endocrine disruptor and reduction in PGC number prior to the first meiosis during sex determination. These findings suggest that a mode of action of perchlorate on adult reproductive phenotypes in vertebrates, including humans, such as altered fecundity and sex reversal or intersex gonads, may stem from early changes to germ cell development.
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spelling pubmed-49348642016-07-18 Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback Petersen, Ann M. Earp, Nathanial C. Redmond, Mandy E. Postlethwait, John H. von Hippel, Frank A. Buck, C. Loren Cresko, William A. PLoS One Research Article Perchlorate is a common aquatic contaminant that has long been known to affect thyroid function in vertebrates, including humans. More recently perchlorate has been shown to affect primordial sexual differentiation in the aquatic model fishes zebrafish and threespine stickleback, but the mechanism has been unclear. Stickleback exposed to perchlorate from fertilization have increased androgen levels in the embryo and disrupted reproductive morphologies as adults, suggesting that perchlorate could disrupt the earliest stages of primordial sexual differentiation when primordial germ cells (PGCs) begin to form the gonad. Female stickleback have three to four times the number of PGCs as males during the first weeks of development. We hypothesized that perchlorate exposure affects primordial sexual differentiation by reducing the number of germ cells in the gonad during an important window of stickleback sex determination at 14–18 days post fertilization (dpf). We tested this hypothesis by quantifying the number of PGCs at 16 dpf in control and 100 mg/L perchlorate-treated male and female stickleback. Perchlorate exposure from the time of fertilization resulted in significantly reduced PGC number only in genotypic females, suggesting that the masculinizing effects of perchlorate observed in adult stickleback may result from early changes to the number of PGCs at a time critical for sex determination. To our knowledge, this is the first evidence of a connection between an endocrine disruptor and reduction in PGC number prior to the first meiosis during sex determination. These findings suggest that a mode of action of perchlorate on adult reproductive phenotypes in vertebrates, including humans, such as altered fecundity and sex reversal or intersex gonads, may stem from early changes to germ cell development. Public Library of Science 2016-07-06 /pmc/articles/PMC4934864/ /pubmed/27383240 http://dx.doi.org/10.1371/journal.pone.0157792 Text en © 2016 Petersen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Petersen, Ann M.
Earp, Nathanial C.
Redmond, Mandy E.
Postlethwait, John H.
von Hippel, Frank A.
Buck, C. Loren
Cresko, William A.
Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback
title Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback
title_full Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback
title_fullStr Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback
title_full_unstemmed Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback
title_short Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback
title_sort perchlorate exposure reduces primordial germ cell number in female threespine stickleback
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4934864/
https://www.ncbi.nlm.nih.gov/pubmed/27383240
http://dx.doi.org/10.1371/journal.pone.0157792
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