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Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB

Elevated levels of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β are often observed in the sera of hepatitis B virus (HBV)-infected patients. It is well known that these cytokines activate nuclear factor-κB (NF-κB)-signaling, and are associated with endoplasm...

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Autores principales: Wu, Shuang, Kanda, Tatsuo, Nakamoto, Shingo, Jiang, Xia, Nakamura, Masato, Sasaki, Reina, Haga, Yuki, Shirasawa, Hiroshi, Yokosuka, Osamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935457/
https://www.ncbi.nlm.nih.gov/pubmed/27315566
http://dx.doi.org/10.3892/ijmm.2016.2643
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author Wu, Shuang
Kanda, Tatsuo
Nakamoto, Shingo
Jiang, Xia
Nakamura, Masato
Sasaki, Reina
Haga, Yuki
Shirasawa, Hiroshi
Yokosuka, Osamu
author_facet Wu, Shuang
Kanda, Tatsuo
Nakamoto, Shingo
Jiang, Xia
Nakamura, Masato
Sasaki, Reina
Haga, Yuki
Shirasawa, Hiroshi
Yokosuka, Osamu
author_sort Wu, Shuang
collection PubMed
description Elevated levels of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β are often observed in the sera of hepatitis B virus (HBV)-infected patients. It is well known that these cytokines activate nuclear factor-κB (NF-κB)-signaling, and are associated with endoplasmic reticulum (ER) stress. We investigated whether HBV or HBV X protein (HBx) enhanced the activation of NF-κB in the presence of TNF and/or IL-1β, and their effects on the expression of metabolic pathway-associated genes. We examined whether HBV or HBx enhanced cytokine-induced activation of NF-κB in hepatocytes, using a reporter assay, in the presence or absence of TNF and/or IL-1β. The expression of insulin-like growth factor binding protein 1 (IGFBP1), one of the NF-κB target genes was also examined. The expression of metabolic pathway-associated genes in HepG2 and HepG2.2.15 cells in the presence or absence of TNF was evaluated by RT-qPCR. Human hepatocytes expressed TNF receptors and IL-1 receptors. NF-κB was activated by cooperation between HBx and TNF in human hepatocytes. We observed IGFBP1 expression in HBV infection and that a number of metabolic pathway-associated genes were upregulated in HepG2.2.15 cells, compared with HepG2 cells with or without TNF treatment. We observed the cooperative effects of HBV and TNF which enhanced the activation of NF-κB as well as upregulated the expression of metabolic pathway-associated genes in hepatocytes. These effects may be important in the development of HBV-associated metabolic syndrome.
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spelling pubmed-49354572016-07-21 Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB Wu, Shuang Kanda, Tatsuo Nakamoto, Shingo Jiang, Xia Nakamura, Masato Sasaki, Reina Haga, Yuki Shirasawa, Hiroshi Yokosuka, Osamu Int J Mol Med Articles Elevated levels of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β are often observed in the sera of hepatitis B virus (HBV)-infected patients. It is well known that these cytokines activate nuclear factor-κB (NF-κB)-signaling, and are associated with endoplasmic reticulum (ER) stress. We investigated whether HBV or HBV X protein (HBx) enhanced the activation of NF-κB in the presence of TNF and/or IL-1β, and their effects on the expression of metabolic pathway-associated genes. We examined whether HBV or HBx enhanced cytokine-induced activation of NF-κB in hepatocytes, using a reporter assay, in the presence or absence of TNF and/or IL-1β. The expression of insulin-like growth factor binding protein 1 (IGFBP1), one of the NF-κB target genes was also examined. The expression of metabolic pathway-associated genes in HepG2 and HepG2.2.15 cells in the presence or absence of TNF was evaluated by RT-qPCR. Human hepatocytes expressed TNF receptors and IL-1 receptors. NF-κB was activated by cooperation between HBx and TNF in human hepatocytes. We observed IGFBP1 expression in HBV infection and that a number of metabolic pathway-associated genes were upregulated in HepG2.2.15 cells, compared with HepG2 cells with or without TNF treatment. We observed the cooperative effects of HBV and TNF which enhanced the activation of NF-κB as well as upregulated the expression of metabolic pathway-associated genes in hepatocytes. These effects may be important in the development of HBV-associated metabolic syndrome. D.A. Spandidos 2016-08 2016-06-16 /pmc/articles/PMC4935457/ /pubmed/27315566 http://dx.doi.org/10.3892/ijmm.2016.2643 Text en Copyright: © Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wu, Shuang
Kanda, Tatsuo
Nakamoto, Shingo
Jiang, Xia
Nakamura, Masato
Sasaki, Reina
Haga, Yuki
Shirasawa, Hiroshi
Yokosuka, Osamu
Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB
title Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB
title_full Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB
title_fullStr Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB
title_full_unstemmed Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB
title_short Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB
title_sort cooperative effects of hepatitis b virus and tnf may play important roles in the activation of metabolic pathways through the activation of nf-κb
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935457/
https://www.ncbi.nlm.nih.gov/pubmed/27315566
http://dx.doi.org/10.3892/ijmm.2016.2643
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