Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores

5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a...

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Detalles Bibliográficos
Autores principales: Zahid, Osama K., Zhao, Boxuan Simen, He, Chuan, Hall, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935868/
https://www.ncbi.nlm.nih.gov/pubmed/27383905
http://dx.doi.org/10.1038/srep29565
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author Zahid, Osama K.
Zhao, Boxuan Simen
He, Chuan
Hall, Adam R.
author_facet Zahid, Osama K.
Zhao, Boxuan Simen
He, Chuan
Hall, Adam R.
author_sort Zahid, Osama K.
collection PubMed
description 5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA.
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spelling pubmed-49358682016-07-08 Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores Zahid, Osama K. Zhao, Boxuan Simen He, Chuan Hall, Adam R. Sci Rep Article 5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA. Nature Publishing Group 2016-07-07 /pmc/articles/PMC4935868/ /pubmed/27383905 http://dx.doi.org/10.1038/srep29565 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zahid, Osama K.
Zhao, Boxuan Simen
He, Chuan
Hall, Adam R.
Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
title Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
title_full Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
title_fullStr Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
title_full_unstemmed Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
title_short Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores
title_sort quantifying mammalian genomic dna hydroxymethylcytosine content using solid-state nanopores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935868/
https://www.ncbi.nlm.nih.gov/pubmed/27383905
http://dx.doi.org/10.1038/srep29565
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