Cargando…
STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection
Mycobacterium tuberculosis is a successful intracellular pathogen. Numerous host innate immune responses signaling pathways are induced upon mycobacterium invasion, however their impact on M. tuberculosis replication is not fully understood. Here we reinvestigate the role of STAT3 specifically insid...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935992/ https://www.ncbi.nlm.nih.gov/pubmed/27384401 http://dx.doi.org/10.1038/srep29297 |
_version_ | 1782441496135335936 |
---|---|
author | Queval, Christophe J. Song, Ok-Ryul Deboosère, Nathalie Delorme, Vincent Debrie, Anne-Sophie Iantomasi, Raffaella Veyron-Churlet, Romain Jouny, Samuel Redhage, Keely Deloison, Gaspard Baulard, Alain Chamaillard, Mathias Locht, Camille Brodin, Priscille |
author_facet | Queval, Christophe J. Song, Ok-Ryul Deboosère, Nathalie Delorme, Vincent Debrie, Anne-Sophie Iantomasi, Raffaella Veyron-Churlet, Romain Jouny, Samuel Redhage, Keely Deloison, Gaspard Baulard, Alain Chamaillard, Mathias Locht, Camille Brodin, Priscille |
author_sort | Queval, Christophe J. |
collection | PubMed |
description | Mycobacterium tuberculosis is a successful intracellular pathogen. Numerous host innate immune responses signaling pathways are induced upon mycobacterium invasion, however their impact on M. tuberculosis replication is not fully understood. Here we reinvestigate the role of STAT3 specifically inside human macrophages shortly after M. tuberculosis uptake. We first show that STAT3 activation is mediated by IL-10 and occurs in M. tuberculosis infected cells as well as in bystander non-colonized cells. STAT3 activation results in the inhibition of IL-6, TNF-α, IFN-γ and MIP-1β. We further demonstrate that STAT3 represses iNOS expression and NO synthesis. Accordingly, the inhibition of STAT3 is detrimental for M. tuberculosis intracellular replication. Our study thus points out STAT3 as a key host factor for M. tuberculosis intracellular establishment in the early stages of macrophage infection. |
format | Online Article Text |
id | pubmed-4935992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49359922016-07-13 STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection Queval, Christophe J. Song, Ok-Ryul Deboosère, Nathalie Delorme, Vincent Debrie, Anne-Sophie Iantomasi, Raffaella Veyron-Churlet, Romain Jouny, Samuel Redhage, Keely Deloison, Gaspard Baulard, Alain Chamaillard, Mathias Locht, Camille Brodin, Priscille Sci Rep Article Mycobacterium tuberculosis is a successful intracellular pathogen. Numerous host innate immune responses signaling pathways are induced upon mycobacterium invasion, however their impact on M. tuberculosis replication is not fully understood. Here we reinvestigate the role of STAT3 specifically inside human macrophages shortly after M. tuberculosis uptake. We first show that STAT3 activation is mediated by IL-10 and occurs in M. tuberculosis infected cells as well as in bystander non-colonized cells. STAT3 activation results in the inhibition of IL-6, TNF-α, IFN-γ and MIP-1β. We further demonstrate that STAT3 represses iNOS expression and NO synthesis. Accordingly, the inhibition of STAT3 is detrimental for M. tuberculosis intracellular replication. Our study thus points out STAT3 as a key host factor for M. tuberculosis intracellular establishment in the early stages of macrophage infection. Nature Publishing Group 2016-07-07 /pmc/articles/PMC4935992/ /pubmed/27384401 http://dx.doi.org/10.1038/srep29297 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Queval, Christophe J. Song, Ok-Ryul Deboosère, Nathalie Delorme, Vincent Debrie, Anne-Sophie Iantomasi, Raffaella Veyron-Churlet, Romain Jouny, Samuel Redhage, Keely Deloison, Gaspard Baulard, Alain Chamaillard, Mathias Locht, Camille Brodin, Priscille STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection |
title | STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection |
title_full | STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection |
title_fullStr | STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection |
title_full_unstemmed | STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection |
title_short | STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection |
title_sort | stat3 represses nitric oxide synthesis in human macrophages upon mycobacterium tuberculosis infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935992/ https://www.ncbi.nlm.nih.gov/pubmed/27384401 http://dx.doi.org/10.1038/srep29297 |
work_keys_str_mv | AT quevalchristophej stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT songokryul stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT debooserenathalie stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT delormevincent stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT debrieannesophie stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT iantomasiraffaella stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT veyronchurletromain stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT jounysamuel stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT redhagekeely stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT deloisongaspard stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT baulardalain stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT chamaillardmathias stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT lochtcamille stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection AT brodinpriscille stat3repressesnitricoxidesynthesisinhumanmacrophagesuponmycobacteriumtuberculosisinfection |