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Establishment of serological test to detect antibody against ferret coronavirus
Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method fo...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937135/ https://www.ncbi.nlm.nih.gov/pubmed/26935842 http://dx.doi.org/10.1292/jvms.16-0059 |
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author | MINAMI, Shohei TERADA, Yutaka SHIMODA, Hiroshi TAKIZAWA, Masaki ONUMA, Mamoru OTA, Akihiko OTA, Yuichi AKABANE, Yoshihito TAMUKAI, Kenichi WATANABE, Keiichiro NAGANUMA, Yumiko KANAGAWA, Eiichi NAKAMURA, Kaneichi OHASHI, Masanari TAKAMI, Yoshinori MIWA, Yasutsugu TANOUE, Tomoaki OHWAKI, Masao OHTA, Jouji UNE, Yumi MAEDA, Ken |
author_facet | MINAMI, Shohei TERADA, Yutaka SHIMODA, Hiroshi TAKIZAWA, Masaki ONUMA, Mamoru OTA, Akihiko OTA, Yuichi AKABANE, Yoshihito TAMUKAI, Kenichi WATANABE, Keiichiro NAGANUMA, Yumiko KANAGAWA, Eiichi NAKAMURA, Kaneichi OHASHI, Masanari TAKAMI, Yoshinori MIWA, Yasutsugu TANOUE, Tomoaki OHWAKI, Masao OHTA, Jouji UNE, Yumi MAEDA, Ken |
author_sort | MINAMI, Shohei |
collection | PubMed |
description | Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV infection. Many serum samples collected from ferrets recognized both a.a. 1–179 and a.a. 180–374 of the N protein, but two serum samples did not a.a. 180–374 of the N protein. This different reactivity was also confirmed by immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1–179 of the N protein was used as an ELISA antigen. Serological test was carried out using sera or plasma of ferrets in Japan. Surprisingly, 89% ferrets in Japan had been infected with FRCoV. These results indicated that our established ELISA using a.a. 1–179 of the N protein is useful for detection of antibody to FRCoV for diagnosis and seroepidemiology of FRCoV infection. |
format | Online Article Text |
id | pubmed-4937135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49371352016-07-11 Establishment of serological test to detect antibody against ferret coronavirus MINAMI, Shohei TERADA, Yutaka SHIMODA, Hiroshi TAKIZAWA, Masaki ONUMA, Mamoru OTA, Akihiko OTA, Yuichi AKABANE, Yoshihito TAMUKAI, Kenichi WATANABE, Keiichiro NAGANUMA, Yumiko KANAGAWA, Eiichi NAKAMURA, Kaneichi OHASHI, Masanari TAKAMI, Yoshinori MIWA, Yasutsugu TANOUE, Tomoaki OHWAKI, Masao OHTA, Jouji UNE, Yumi MAEDA, Ken J Vet Med Sci Virology Since there is no available serological methods to detect antibodies to ferret coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) Yamaguchi-1 strain was developed to establish a serological method for detection of FRCoV infection. Many serum samples collected from ferrets recognized both a.a. 1–179 and a.a. 180–374 of the N protein, but two serum samples did not a.a. 180–374 of the N protein. This different reactivity was also confirmed by immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1–179 of the N protein was used as an ELISA antigen. Serological test was carried out using sera or plasma of ferrets in Japan. Surprisingly, 89% ferrets in Japan had been infected with FRCoV. These results indicated that our established ELISA using a.a. 1–179 of the N protein is useful for detection of antibody to FRCoV for diagnosis and seroepidemiology of FRCoV infection. The Japanese Society of Veterinary Science 2016-03-03 2016-06 /pmc/articles/PMC4937135/ /pubmed/26935842 http://dx.doi.org/10.1292/jvms.16-0059 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Virology MINAMI, Shohei TERADA, Yutaka SHIMODA, Hiroshi TAKIZAWA, Masaki ONUMA, Mamoru OTA, Akihiko OTA, Yuichi AKABANE, Yoshihito TAMUKAI, Kenichi WATANABE, Keiichiro NAGANUMA, Yumiko KANAGAWA, Eiichi NAKAMURA, Kaneichi OHASHI, Masanari TAKAMI, Yoshinori MIWA, Yasutsugu TANOUE, Tomoaki OHWAKI, Masao OHTA, Jouji UNE, Yumi MAEDA, Ken Establishment of serological test to detect antibody against ferret coronavirus |
title | Establishment of serological test to detect antibody against ferret coronavirus |
title_full | Establishment of serological test to detect antibody against ferret coronavirus |
title_fullStr | Establishment of serological test to detect antibody against ferret coronavirus |
title_full_unstemmed | Establishment of serological test to detect antibody against ferret coronavirus |
title_short | Establishment of serological test to detect antibody against ferret coronavirus |
title_sort | establishment of serological test to detect antibody against ferret coronavirus |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937135/ https://www.ncbi.nlm.nih.gov/pubmed/26935842 http://dx.doi.org/10.1292/jvms.16-0059 |
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