Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries

The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut microbiota of laboratory mice. The 566 clones were obtained from the clone libraries generated from the fecal DNA samples...

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Autores principales: NOZU, Ryoko, UENO, Masami, HAYASHIMOTO, Nobuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Laboratory Animal Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937141/
https://www.ncbi.nlm.nih.gov/pubmed/26902692
http://dx.doi.org/10.1292/jvms.15-0454
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author NOZU, Ryoko
UENO, Masami
HAYASHIMOTO, Nobuhito
author_facet NOZU, Ryoko
UENO, Masami
HAYASHIMOTO, Nobuhito
author_sort NOZU, Ryoko
collection PubMed
description The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut microbiota of laboratory mice. The 566 clones were obtained from the clone libraries generated from the fecal DNA samples derived from BALB/c, C57BL/6N, DBA/2 and ICR mice. Among these 566 clones, there were 446 unique 16S rRNA gene sequences. When grouped at the 98% similarity level, the 446 unique sequences consisted of 103 Clostridiales, 43 Bacteroidales, 5 Lactobacillus and 3 Erysipelotricaceae, as well as sequences from 11 other phyla.
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spelling pubmed-49371412016-07-11 Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries NOZU, Ryoko UENO, Masami HAYASHIMOTO, Nobuhito J Vet Med Sci Laboratory Animal Science The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut microbiota of laboratory mice. The 566 clones were obtained from the clone libraries generated from the fecal DNA samples derived from BALB/c, C57BL/6N, DBA/2 and ICR mice. Among these 566 clones, there were 446 unique 16S rRNA gene sequences. When grouped at the 98% similarity level, the 446 unique sequences consisted of 103 Clostridiales, 43 Bacteroidales, 5 Lactobacillus and 3 Erysipelotricaceae, as well as sequences from 11 other phyla. The Japanese Society of Veterinary Science 2016-02-20 2016-06 /pmc/articles/PMC4937141/ /pubmed/26902692 http://dx.doi.org/10.1292/jvms.15-0454 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Laboratory Animal Science
NOZU, Ryoko
UENO, Masami
HAYASHIMOTO, Nobuhito
Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
title Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
title_full Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
title_fullStr Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
title_full_unstemmed Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
title_short Composition of fecal microbiota of laboratory mice derived from Japanese commercial breeders using 16S rRNA gene clone libraries
title_sort composition of fecal microbiota of laboratory mice derived from japanese commercial breeders using 16s rrna gene clone libraries
topic Laboratory Animal Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937141/
https://www.ncbi.nlm.nih.gov/pubmed/26902692
http://dx.doi.org/10.1292/jvms.15-0454
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