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Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders

The activity of matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fluids (SF) sampled from dogs with joint disorders was investigated by gelatin zymography and densitometry. Pro-MMP-2 showed similar activity levels in dogs with idiopathic polyarthritis (IPA; n=17) or canine rheumatoid arthritis...

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Autores principales: MURAKAMI, Kohei, MAEDA, Shingo, YONEZAWA, Tomohiro, MATSUKI, Naoaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937142/
https://www.ncbi.nlm.nih.gov/pubmed/26902805
http://dx.doi.org/10.1292/jvms.15-0711
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author MURAKAMI, Kohei
MAEDA, Shingo
YONEZAWA, Tomohiro
MATSUKI, Naoaki
author_facet MURAKAMI, Kohei
MAEDA, Shingo
YONEZAWA, Tomohiro
MATSUKI, Naoaki
author_sort MURAKAMI, Kohei
collection PubMed
description The activity of matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fluids (SF) sampled from dogs with joint disorders was investigated by gelatin zymography and densitometry. Pro-MMP-2 showed similar activity levels in dogs with idiopathic polyarthritis (IPA; n=17) or canine rheumatoid arthritis (cRA; n=4), and healthy controls (n=10). However, dogs with cranial cruciate ligament rupture (CCLR; n=5) presented significantly higher pro-MMP-2 activity than IPA and healthy dogs. Meanwhile, dogs with IPA exhibited significantly higher activity of pro- and active MMP-9 than other groups. Activity levels in pro- and active MMP-9 in cRA and CCLR dogs were not significantly different from those in healthy controls. Different patterns of MMP-2 and MMP-9 activity may reflect the differences in the underlying pathological processes.
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spelling pubmed-49371422016-07-11 Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders MURAKAMI, Kohei MAEDA, Shingo YONEZAWA, Tomohiro MATSUKI, Naoaki J Vet Med Sci Clinical Pathology The activity of matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fluids (SF) sampled from dogs with joint disorders was investigated by gelatin zymography and densitometry. Pro-MMP-2 showed similar activity levels in dogs with idiopathic polyarthritis (IPA; n=17) or canine rheumatoid arthritis (cRA; n=4), and healthy controls (n=10). However, dogs with cranial cruciate ligament rupture (CCLR; n=5) presented significantly higher pro-MMP-2 activity than IPA and healthy dogs. Meanwhile, dogs with IPA exhibited significantly higher activity of pro- and active MMP-9 than other groups. Activity levels in pro- and active MMP-9 in cRA and CCLR dogs were not significantly different from those in healthy controls. Different patterns of MMP-2 and MMP-9 activity may reflect the differences in the underlying pathological processes. The Japanese Society of Veterinary Science 2016-02-22 2016-06 /pmc/articles/PMC4937142/ /pubmed/26902805 http://dx.doi.org/10.1292/jvms.15-0711 Text en ©2016 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Clinical Pathology
MURAKAMI, Kohei
MAEDA, Shingo
YONEZAWA, Tomohiro
MATSUKI, Naoaki
Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
title Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
title_full Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
title_fullStr Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
title_full_unstemmed Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
title_short Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
title_sort synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders
topic Clinical Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937142/
https://www.ncbi.nlm.nih.gov/pubmed/26902805
http://dx.doi.org/10.1292/jvms.15-0711
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