Global Membrane Protein Interactome Analysis using In vivo Crosslinking and Mass Spectrometry-based Protein Correlation Profiling

We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...

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Detalles Bibliográficos
Autores principales: Larance, Mark, Kirkwood, Kathryn J., Tinti, Michele, Brenes Murillo, Alejandro, Ferguson, Michael A. J., Lamond, Angus I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2016
Materias:
Technological Innovation and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937518/
https://www.ncbi.nlm.nih.gov/pubmed/27114452
http://dx.doi.org/10.1074/mcp.O115.055467

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4937518/
https://www.ncbi.nlm.nih.gov/pubmed/27114452
http://dx.doi.org/10.1074/mcp.O115.055467

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