LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo

The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and ove...

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Autores principales: Chen, Long, Yang, Huixin, Xiao, Yanbin, Tang, Xiaoxia, Li, Yuqian, Han, Qiaoqiao, Fu, Junping, Yang, Yuye, Zhu, Yuechun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938146/
https://www.ncbi.nlm.nih.gov/pubmed/27445498
http://dx.doi.org/10.2147/OTT.S98203
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author Chen, Long
Yang, Huixin
Xiao, Yanbin
Tang, Xiaoxia
Li, Yuqian
Han, Qiaoqiao
Fu, Junping
Yang, Yuye
Zhu, Yuechun
author_facet Chen, Long
Yang, Huixin
Xiao, Yanbin
Tang, Xiaoxia
Li, Yuqian
Han, Qiaoqiao
Fu, Junping
Yang, Yuye
Zhu, Yuechun
author_sort Chen, Long
collection PubMed
description The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo.
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spelling pubmed-49381462016-07-21 LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo Chen, Long Yang, Huixin Xiao, Yanbin Tang, Xiaoxia Li, Yuqian Han, Qiaoqiao Fu, Junping Yang, Yuye Zhu, Yuechun Onco Targets Ther Original Research The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo. Dove Medical Press 2016-07-04 /pmc/articles/PMC4938146/ /pubmed/27445498 http://dx.doi.org/10.2147/OTT.S98203 Text en © 2016 Chen et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Chen, Long
Yang, Huixin
Xiao, Yanbin
Tang, Xiaoxia
Li, Yuqian
Han, Qiaoqiao
Fu, Junping
Yang, Yuye
Zhu, Yuechun
LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
title LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
title_full LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
title_fullStr LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
title_full_unstemmed LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
title_short LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
title_sort lncrna gas5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938146/
https://www.ncbi.nlm.nih.gov/pubmed/27445498
http://dx.doi.org/10.2147/OTT.S98203
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