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Multiplexed Spectral Imaging of 120 Different Fluorescent Labels

The number of fluorescent labels that can unambiguously be distinguished in a single image when acquired through band pass filters is severely limited by the spectral overlap of available fluorophores. The recent development of spectral microscopy and the application of linear unmixing algorithms to...

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Detalles Bibliográficos
Autores principales: Valm, Alex M., Oldenbourg, Rudolf, Borisy, Gary G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938436/
https://www.ncbi.nlm.nih.gov/pubmed/27391327
http://dx.doi.org/10.1371/journal.pone.0158495