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Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes

Hybridization plays an important role in fish breeding. Bream fishes contribute a lot to aquaculture in China due to their economically valuable characteristics and the present study included five bream species, Megalobrama amblycephala, Megalobrama skolkovii, Megalobrama pellegrini, Megalobrama ter...

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Autores principales: Zhang, Wei-Zhuo, Xiong, Xue-Mei, Zhang, Xiu-Jie, Wan, Shi-Ming, Guan, Ning-Nan, Nie, Chun-Hong, Zhao, Bo-Wen, Hsiao, Chung-Der, Wang, Wei-Min, Gao, Ze-Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938612/
https://www.ncbi.nlm.nih.gov/pubmed/27391325
http://dx.doi.org/10.1371/journal.pone.0158915
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author Zhang, Wei-Zhuo
Xiong, Xue-Mei
Zhang, Xiu-Jie
Wan, Shi-Ming
Guan, Ning-Nan
Nie, Chun-Hong
Zhao, Bo-Wen
Hsiao, Chung-Der
Wang, Wei-Min
Gao, Ze-Xia
author_facet Zhang, Wei-Zhuo
Xiong, Xue-Mei
Zhang, Xiu-Jie
Wan, Shi-Ming
Guan, Ning-Nan
Nie, Chun-Hong
Zhao, Bo-Wen
Hsiao, Chung-Der
Wang, Wei-Min
Gao, Ze-Xia
author_sort Zhang, Wei-Zhuo
collection PubMed
description Hybridization plays an important role in fish breeding. Bream fishes contribute a lot to aquaculture in China due to their economically valuable characteristics and the present study included five bream species, Megalobrama amblycephala, Megalobrama skolkovii, Megalobrama pellegrini, Megalobrama terminalis and Parabramis pekinensis. As maternal inheritance of mitochondrial genome (mitogenome) involves species specific regulation, we aimed to investigate in which way the inheritance of mitogenome is affected by hybridization in these fish species. With complete mitogenomes of 7 hybrid groups of bream species being firstly reported in the present study, a comparative analysis of 17 mitogenomes was conducted, including representatives of these 5 bream species, 6 first generation hybrids and 6 second generation hybrids. The results showed that these 17 mitogenomes shared the same gene arrangement, and had similar gene size and base composition. According to the phylogenetic analyses, all mitogenomes of the hybrids were consistent with a maternal inheritance. However, a certain number of variable sites were detected in all F(1) hybrid groups compared to their female parents, especially in the group of M. terminalis (♀) × M. amblycephala (♂) (MT×MA), with a total of 86 variable sites between MT×MA and its female parent. Among the mitogenomes genes, the protein-coding gene nd5 displayed the highest variability. The number of variation sites was found to be related to phylogenetic relationship of the parents: the closer they are, the lower amount of variation sites their hybrids have. The second generation hybrids showed less mitogenome variation than that of first generation hybrids. The non-synonymous and synonymous substitution rates (dN/dS) were calculated between all the hybrids with their own female parents and the results indicated that most PCGs were under negative selection.
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spelling pubmed-49386122016-07-22 Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes Zhang, Wei-Zhuo Xiong, Xue-Mei Zhang, Xiu-Jie Wan, Shi-Ming Guan, Ning-Nan Nie, Chun-Hong Zhao, Bo-Wen Hsiao, Chung-Der Wang, Wei-Min Gao, Ze-Xia PLoS One Research Article Hybridization plays an important role in fish breeding. Bream fishes contribute a lot to aquaculture in China due to their economically valuable characteristics and the present study included five bream species, Megalobrama amblycephala, Megalobrama skolkovii, Megalobrama pellegrini, Megalobrama terminalis and Parabramis pekinensis. As maternal inheritance of mitochondrial genome (mitogenome) involves species specific regulation, we aimed to investigate in which way the inheritance of mitogenome is affected by hybridization in these fish species. With complete mitogenomes of 7 hybrid groups of bream species being firstly reported in the present study, a comparative analysis of 17 mitogenomes was conducted, including representatives of these 5 bream species, 6 first generation hybrids and 6 second generation hybrids. The results showed that these 17 mitogenomes shared the same gene arrangement, and had similar gene size and base composition. According to the phylogenetic analyses, all mitogenomes of the hybrids were consistent with a maternal inheritance. However, a certain number of variable sites were detected in all F(1) hybrid groups compared to their female parents, especially in the group of M. terminalis (♀) × M. amblycephala (♂) (MT×MA), with a total of 86 variable sites between MT×MA and its female parent. Among the mitogenomes genes, the protein-coding gene nd5 displayed the highest variability. The number of variation sites was found to be related to phylogenetic relationship of the parents: the closer they are, the lower amount of variation sites their hybrids have. The second generation hybrids showed less mitogenome variation than that of first generation hybrids. The non-synonymous and synonymous substitution rates (dN/dS) were calculated between all the hybrids with their own female parents and the results indicated that most PCGs were under negative selection. Public Library of Science 2016-07-08 /pmc/articles/PMC4938612/ /pubmed/27391325 http://dx.doi.org/10.1371/journal.pone.0158915 Text en © 2016 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Wei-Zhuo
Xiong, Xue-Mei
Zhang, Xiu-Jie
Wan, Shi-Ming
Guan, Ning-Nan
Nie, Chun-Hong
Zhao, Bo-Wen
Hsiao, Chung-Der
Wang, Wei-Min
Gao, Ze-Xia
Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes
title Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes
title_full Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes
title_fullStr Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes
title_full_unstemmed Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes
title_short Mitochondrial Genome Variation after Hybridization and Differences in the First and Second Generation Hybrids of Bream Fishes
title_sort mitochondrial genome variation after hybridization and differences in the first and second generation hybrids of bream fishes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4938612/
https://www.ncbi.nlm.nih.gov/pubmed/27391325
http://dx.doi.org/10.1371/journal.pone.0158915
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