Cargando…
Stachybotrys mycotoxins: from culture extracts to dust samples
The filamentous fungus Stachybotrys chartarum is known for its toxic metabolites and has been associated with serious health problems, including mycotoxicosis, among occupants of contaminated buildings. Here, we present results from a case study, where an ultra-high performance liquid chromatography...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4939167/ https://www.ncbi.nlm.nih.gov/pubmed/27255106 http://dx.doi.org/10.1007/s00216-016-9649-y |
_version_ | 1782441964179816448 |
---|---|
author | Došen, Ina Andersen, Birgitte Phippen, Christopher B. W. Clausen, Geo Nielsen, Kristian Fog |
author_facet | Došen, Ina Andersen, Birgitte Phippen, Christopher B. W. Clausen, Geo Nielsen, Kristian Fog |
author_sort | Došen, Ina |
collection | PubMed |
description | The filamentous fungus Stachybotrys chartarum is known for its toxic metabolites and has been associated with serious health problems, including mycotoxicosis, among occupants of contaminated buildings. Here, we present results from a case study, where an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for known and tentatively identified compounds characterized via UHPLC-quadruple time-of-flight (QTOF) screening of fungal culture extracts, wall scrapings and reference standards. The UHPLC-MS/MS method was able to identify 12 Stachybotrys metabolites, of which four could be quantified based on authentic standards and a further six estimated based on similarity to authentic standards. Samples collected from walls contaminated by S. chartarum in a water-damaged building showed that the two known chemotypes, S and A, coexisted. More importantly, a link between mycotoxin concentrations found on contaminated surfaces and in settled dust was made. One dust sample, collected from a water-damaged room, contained 10 pg/cm(2) macrocyclic trichothecenes (roridin E). For the first time, more than one spirocyclic drimane was detected in dust. Spirocyclic drimanes were detected in all 11 analysed dust samples and in total amounted to 600 pg/cm(2) in the water-damaged room and 340 pg/cm(2) in rooms adjacent to the water-damaged area. Their wide distribution in detectable amounts in dust suggested they could be good candidates for exposure biomarkers. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-016-9649-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4939167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-49391672016-07-21 Stachybotrys mycotoxins: from culture extracts to dust samples Došen, Ina Andersen, Birgitte Phippen, Christopher B. W. Clausen, Geo Nielsen, Kristian Fog Anal Bioanal Chem Research Paper The filamentous fungus Stachybotrys chartarum is known for its toxic metabolites and has been associated with serious health problems, including mycotoxicosis, among occupants of contaminated buildings. Here, we present results from a case study, where an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for known and tentatively identified compounds characterized via UHPLC-quadruple time-of-flight (QTOF) screening of fungal culture extracts, wall scrapings and reference standards. The UHPLC-MS/MS method was able to identify 12 Stachybotrys metabolites, of which four could be quantified based on authentic standards and a further six estimated based on similarity to authentic standards. Samples collected from walls contaminated by S. chartarum in a water-damaged building showed that the two known chemotypes, S and A, coexisted. More importantly, a link between mycotoxin concentrations found on contaminated surfaces and in settled dust was made. One dust sample, collected from a water-damaged room, contained 10 pg/cm(2) macrocyclic trichothecenes (roridin E). For the first time, more than one spirocyclic drimane was detected in dust. Spirocyclic drimanes were detected in all 11 analysed dust samples and in total amounted to 600 pg/cm(2) in the water-damaged room and 340 pg/cm(2) in rooms adjacent to the water-damaged area. Their wide distribution in detectable amounts in dust suggested they could be good candidates for exposure biomarkers. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-016-9649-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-06-02 2016 /pmc/articles/PMC4939167/ /pubmed/27255106 http://dx.doi.org/10.1007/s00216-016-9649-y Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Paper Došen, Ina Andersen, Birgitte Phippen, Christopher B. W. Clausen, Geo Nielsen, Kristian Fog Stachybotrys mycotoxins: from culture extracts to dust samples |
title | Stachybotrys mycotoxins: from culture extracts to dust samples |
title_full | Stachybotrys mycotoxins: from culture extracts to dust samples |
title_fullStr | Stachybotrys mycotoxins: from culture extracts to dust samples |
title_full_unstemmed | Stachybotrys mycotoxins: from culture extracts to dust samples |
title_short | Stachybotrys mycotoxins: from culture extracts to dust samples |
title_sort | stachybotrys mycotoxins: from culture extracts to dust samples |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4939167/ https://www.ncbi.nlm.nih.gov/pubmed/27255106 http://dx.doi.org/10.1007/s00216-016-9649-y |
work_keys_str_mv | AT dosenina stachybotrysmycotoxinsfromcultureextractstodustsamples AT andersenbirgitte stachybotrysmycotoxinsfromcultureextractstodustsamples AT phippenchristopherbw stachybotrysmycotoxinsfromcultureextractstodustsamples AT clausengeo stachybotrysmycotoxinsfromcultureextractstodustsamples AT nielsenkristianfog stachybotrysmycotoxinsfromcultureextractstodustsamples |