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Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition

Conjugated linoleic acids (CLA) are positional and geometric isomers of linoleic acid involved in a number of health aspects. In humans, CLA production is performed by gut microbiota, including some species of potential probiotic bifidobacteria. 128 strains of 31 Bifidobacterium species were screene...

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Autores principales: Raimondi, Stefano, Amaretti, Alberto, Leonardi, Alan, Quartieri, Andrea, Gozzoli, Caterina, Rossi, Maddalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4939342/
https://www.ncbi.nlm.nih.gov/pubmed/27429985
http://dx.doi.org/10.1155/2016/8654317
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author Raimondi, Stefano
Amaretti, Alberto
Leonardi, Alan
Quartieri, Andrea
Gozzoli, Caterina
Rossi, Maddalena
author_facet Raimondi, Stefano
Amaretti, Alberto
Leonardi, Alan
Quartieri, Andrea
Gozzoli, Caterina
Rossi, Maddalena
author_sort Raimondi, Stefano
collection PubMed
description Conjugated linoleic acids (CLA) are positional and geometric isomers of linoleic acid involved in a number of health aspects. In humans, CLA production is performed by gut microbiota, including some species of potential probiotic bifidobacteria. 128 strains of 31 Bifidobacterium species were screened with a spectrophotometric assay to identify novel CLA producers. Most species were nonproducers, while producers belonged to B. breve and B. pseudocatenulatum. GC-MS revealed that CLA producer strains yielded 9cis,11trans-CLA and 9trans,11trans-CLA, without any production of other isomers. Hydroxylated forms of LA were absent in producer strains, suggesting that the myosin-cross-reactive antigen (MCRA) protein that exerts hydratase activity is not involved in LA isomerization. Moreover, both CLA producer and nonproducer species bear a MCRA homologue. The strain B. breve WC 0421 was the best CLA producer, converting LA into 68.8% 9cis,11trans-CLA and 25.1% 9trans,11trans-CLA. Production occurred mostly during the lag and the exponential phase. For the first time, production and incorporation of CLA in biomass were assessed. B. breve WC 0421 stored CLA in the form of free fatty acids, without changing the composition of the esterified fatty acids, which mainly occurred in the plasmatic membrane.
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spelling pubmed-49393422016-07-17 Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition Raimondi, Stefano Amaretti, Alberto Leonardi, Alan Quartieri, Andrea Gozzoli, Caterina Rossi, Maddalena Biomed Res Int Research Article Conjugated linoleic acids (CLA) are positional and geometric isomers of linoleic acid involved in a number of health aspects. In humans, CLA production is performed by gut microbiota, including some species of potential probiotic bifidobacteria. 128 strains of 31 Bifidobacterium species were screened with a spectrophotometric assay to identify novel CLA producers. Most species were nonproducers, while producers belonged to B. breve and B. pseudocatenulatum. GC-MS revealed that CLA producer strains yielded 9cis,11trans-CLA and 9trans,11trans-CLA, without any production of other isomers. Hydroxylated forms of LA were absent in producer strains, suggesting that the myosin-cross-reactive antigen (MCRA) protein that exerts hydratase activity is not involved in LA isomerization. Moreover, both CLA producer and nonproducer species bear a MCRA homologue. The strain B. breve WC 0421 was the best CLA producer, converting LA into 68.8% 9cis,11trans-CLA and 25.1% 9trans,11trans-CLA. Production occurred mostly during the lag and the exponential phase. For the first time, production and incorporation of CLA in biomass were assessed. B. breve WC 0421 stored CLA in the form of free fatty acids, without changing the composition of the esterified fatty acids, which mainly occurred in the plasmatic membrane. Hindawi Publishing Corporation 2016 2016-06-27 /pmc/articles/PMC4939342/ /pubmed/27429985 http://dx.doi.org/10.1155/2016/8654317 Text en Copyright © 2016 Stefano Raimondi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Raimondi, Stefano
Amaretti, Alberto
Leonardi, Alan
Quartieri, Andrea
Gozzoli, Caterina
Rossi, Maddalena
Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition
title Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition
title_full Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition
title_fullStr Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition
title_full_unstemmed Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition
title_short Conjugated Linoleic Acid Production by Bifidobacteria: Screening, Kinetic, and Composition
title_sort conjugated linoleic acid production by bifidobacteria: screening, kinetic, and composition
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4939342/
https://www.ncbi.nlm.nih.gov/pubmed/27429985
http://dx.doi.org/10.1155/2016/8654317
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