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The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats

BACKGROUND: Labdane-type diterpenes induce lower blood pressure via relaxation of vascular smooth muscle; however, there are no studies describing the effects of labdanes in hypertensive rats. OBJECTIVE: The present study was designed to investigate the cardiovascular actions of the labdane-type dit...

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Autores principales: Simplicio, Janaina A., Simão, Marilia R., Ambrosio, Sergio R., Tirapelli, Carlos R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Cardiologia - SBC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940147/
https://www.ncbi.nlm.nih.gov/pubmed/27096521
http://dx.doi.org/10.5935/abc.20160058
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author Simplicio, Janaina A.
Simão, Marilia R.
Ambrosio, Sergio R.
Tirapelli, Carlos R.
author_facet Simplicio, Janaina A.
Simão, Marilia R.
Ambrosio, Sergio R.
Tirapelli, Carlos R.
author_sort Simplicio, Janaina A.
collection PubMed
description BACKGROUND: Labdane-type diterpenes induce lower blood pressure via relaxation of vascular smooth muscle; however, there are no studies describing the effects of labdanes in hypertensive rats. OBJECTIVE: The present study was designed to investigate the cardiovascular actions of the labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid (labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension. METHODS: Vascular reactivity experiments were performed in aortic rings isolated from 2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx) measurement was performed in aortas by colorimetric assay. Blood pressure measurements were performed in conscious rats. RESULTS: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1 nmol/l - 1 µmol/l) relaxed endothelium-intact and endothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acid was more effective at inducing relaxation in endothelium-intact aortas from 2K pre-contracted with phenylephrine when compared to the endothelium-denuded ones. Forskolin was more potent than labda-15-oic acid at inducing vascular relaxation in arteries from both 2K and 2K-1C rats. Labda-15-oic acid-induced increase in NOx levels was lower in arteries from 2K-1C rats when compared to 2K rats. Intravenous administration of labda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) induced hypotension in conscious 2K-1C and 2K rats. CONCLUSION: The present findings show that labda-15-oic acid induces vascular relaxation and hypotension in hypertensive rats.
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spelling pubmed-49401472016-07-13 The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats Simplicio, Janaina A. Simão, Marilia R. Ambrosio, Sergio R. Tirapelli, Carlos R. Arq Bras Cardiol Original Articles BACKGROUND: Labdane-type diterpenes induce lower blood pressure via relaxation of vascular smooth muscle; however, there are no studies describing the effects of labdanes in hypertensive rats. OBJECTIVE: The present study was designed to investigate the cardiovascular actions of the labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid (labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension. METHODS: Vascular reactivity experiments were performed in aortic rings isolated from 2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx) measurement was performed in aortas by colorimetric assay. Blood pressure measurements were performed in conscious rats. RESULTS: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1 nmol/l - 1 µmol/l) relaxed endothelium-intact and endothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acid was more effective at inducing relaxation in endothelium-intact aortas from 2K pre-contracted with phenylephrine when compared to the endothelium-denuded ones. Forskolin was more potent than labda-15-oic acid at inducing vascular relaxation in arteries from both 2K and 2K-1C rats. Labda-15-oic acid-induced increase in NOx levels was lower in arteries from 2K-1C rats when compared to 2K rats. Intravenous administration of labda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) induced hypotension in conscious 2K-1C and 2K rats. CONCLUSION: The present findings show that labda-15-oic acid induces vascular relaxation and hypotension in hypertensive rats. Sociedade Brasileira de Cardiologia - SBC 2016-06 /pmc/articles/PMC4940147/ /pubmed/27096521 http://dx.doi.org/10.5935/abc.20160058 Text en http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Simplicio, Janaina A.
Simão, Marilia R.
Ambrosio, Sergio R.
Tirapelli, Carlos R.
The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats
title The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats
title_full The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats
title_fullStr The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats
title_full_unstemmed The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats
title_short The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases Blood Pressure In Hypertensive Rats
title_sort labdane ent-3-acetoxy-labda-8(17), 13-dien-15-oic decreases blood pressure in hypertensive rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940147/
https://www.ncbi.nlm.nih.gov/pubmed/27096521
http://dx.doi.org/10.5935/abc.20160058
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