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Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice

We discovered that optical stimulation of the mystacial pad in Emx1-Cre;Ai27D transgenic mice induces whisker movements due to activation of ChR2 expressed in muscles controlling retraction and protraction. Using high-speed videography in anesthetized mice, we characterize the amplitude of whisker p...

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Autores principales: Park, Sunmee, Bandi, Akhil, Lee, Christian R, Margolis, David J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940159/
https://www.ncbi.nlm.nih.gov/pubmed/27269285
http://dx.doi.org/10.7554/eLife.14140
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author Park, Sunmee
Bandi, Akhil
Lee, Christian R
Margolis, David J
author_facet Park, Sunmee
Bandi, Akhil
Lee, Christian R
Margolis, David J
author_sort Park, Sunmee
collection PubMed
description We discovered that optical stimulation of the mystacial pad in Emx1-Cre;Ai27D transgenic mice induces whisker movements due to activation of ChR2 expressed in muscles controlling retraction and protraction. Using high-speed videography in anesthetized mice, we characterize the amplitude of whisker protractions evoked by varying the intensity, duration, and frequency of optogenetic stimulation. Recordings from primary somatosensory cortex (S1) in anesthetized mice indicated that optogenetic whisker pad stimulation evokes robust yet longer latency responses than mechanical whisker stimulation. In head-fixed mice trained to report optogenetic whisker pad stimulation, psychometric curves showed similar dependence on stimulus duration as evoked whisker movements and S1 activity. Furthermore, optogenetic stimulation of S1 in expert mice was sufficient to substitute for peripheral stimulation. We conclude that whisker protractions evoked by optogenetic activation of whisker pad muscles results in cortical activity and sensory perception, consistent with the coding of evoked whisker movements by reafferent sensory input. DOI: http://dx.doi.org/10.7554/eLife.14140.001
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spelling pubmed-49401592016-07-13 Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice Park, Sunmee Bandi, Akhil Lee, Christian R Margolis, David J eLife Neuroscience We discovered that optical stimulation of the mystacial pad in Emx1-Cre;Ai27D transgenic mice induces whisker movements due to activation of ChR2 expressed in muscles controlling retraction and protraction. Using high-speed videography in anesthetized mice, we characterize the amplitude of whisker protractions evoked by varying the intensity, duration, and frequency of optogenetic stimulation. Recordings from primary somatosensory cortex (S1) in anesthetized mice indicated that optogenetic whisker pad stimulation evokes robust yet longer latency responses than mechanical whisker stimulation. In head-fixed mice trained to report optogenetic whisker pad stimulation, psychometric curves showed similar dependence on stimulus duration as evoked whisker movements and S1 activity. Furthermore, optogenetic stimulation of S1 in expert mice was sufficient to substitute for peripheral stimulation. We conclude that whisker protractions evoked by optogenetic activation of whisker pad muscles results in cortical activity and sensory perception, consistent with the coding of evoked whisker movements by reafferent sensory input. DOI: http://dx.doi.org/10.7554/eLife.14140.001 eLife Sciences Publications, Ltd 2016-06-08 /pmc/articles/PMC4940159/ /pubmed/27269285 http://dx.doi.org/10.7554/eLife.14140 Text en © 2016, Park et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Park, Sunmee
Bandi, Akhil
Lee, Christian R
Margolis, David J
Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
title Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
title_full Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
title_fullStr Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
title_full_unstemmed Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
title_short Peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
title_sort peripheral optogenetic stimulation induces whisker movement and sensory perception in head-fixed mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940159/
https://www.ncbi.nlm.nih.gov/pubmed/27269285
http://dx.doi.org/10.7554/eLife.14140
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