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Influenza virus assays based on virus‐inducible reporter cell lines
Background Virus‐inducible reporter genes have been used as the basis of virus detection and quantitation assays for a number of viruses. A strategy for influenza A virus‐induction of a reporter gene was recently described. In this report, we describe the extension of this strategy to influenza B v...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940803/ https://www.ncbi.nlm.nih.gov/pubmed/21462401 http://dx.doi.org/10.1111/j.1750-2659.2009.00095.x |
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author | Li, Yunsheng Larrimer, Audrey Curtiss, Teresa Kim, Jaekyung Jones, Abby Baird‐Tomlinson, Heather Pekosz, Andrew Olivo, Paul D. |
author_facet | Li, Yunsheng Larrimer, Audrey Curtiss, Teresa Kim, Jaekyung Jones, Abby Baird‐Tomlinson, Heather Pekosz, Andrew Olivo, Paul D. |
author_sort | Li, Yunsheng |
collection | PubMed |
description | Background Virus‐inducible reporter genes have been used as the basis of virus detection and quantitation assays for a number of viruses. A strategy for influenza A virus‐induction of a reporter gene was recently described. In this report, we describe the extension of this strategy to influenza B virus, the generation of stable cell lines with influenza A and B virus‐inducible reporter genes, and the use of these cells in various clinically relevant viral assays. Each of the cell lines described herein constitutively express an RNA transcript that contains a reporter gene coding region flanked by viral 5′‐ and 3′‐untranslated regions (UTR) and therefore mimics an influenza virus genomic segment. Upon infection of the cells with influenza virus the virus‐inducible reporter gene segment (VIRGS) is replicated and transcribed by the viral polymerase complex resulting in reporter gene expression. Findings Reporter gene induction occurs after infection with a number of laboratory strains and clinical isolates of influenza virus including several H5N1 strains. The induction is dose‐dependent and highly specific for influenza A or influenza B viruses. Conclusions These cell lines provide the basis of simple, rapid, and objective assays that involve virus quantitation such as determination of viral titer, assessment of antiviral susceptibility, and determination of antibody neutralization titer. These cell lines could be very useful for influenza virus researchers and vaccine manufacturers. |
format | Online Article Text |
id | pubmed-4940803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-49408032016-07-20 Influenza virus assays based on virus‐inducible reporter cell lines Li, Yunsheng Larrimer, Audrey Curtiss, Teresa Kim, Jaekyung Jones, Abby Baird‐Tomlinson, Heather Pekosz, Andrew Olivo, Paul D. Influenza Other Respir Viruses Original Articles Background Virus‐inducible reporter genes have been used as the basis of virus detection and quantitation assays for a number of viruses. A strategy for influenza A virus‐induction of a reporter gene was recently described. In this report, we describe the extension of this strategy to influenza B virus, the generation of stable cell lines with influenza A and B virus‐inducible reporter genes, and the use of these cells in various clinically relevant viral assays. Each of the cell lines described herein constitutively express an RNA transcript that contains a reporter gene coding region flanked by viral 5′‐ and 3′‐untranslated regions (UTR) and therefore mimics an influenza virus genomic segment. Upon infection of the cells with influenza virus the virus‐inducible reporter gene segment (VIRGS) is replicated and transcribed by the viral polymerase complex resulting in reporter gene expression. Findings Reporter gene induction occurs after infection with a number of laboratory strains and clinical isolates of influenza virus including several H5N1 strains. The induction is dose‐dependent and highly specific for influenza A or influenza B viruses. Conclusions These cell lines provide the basis of simple, rapid, and objective assays that involve virus quantitation such as determination of viral titer, assessment of antiviral susceptibility, and determination of antibody neutralization titer. These cell lines could be very useful for influenza virus researchers and vaccine manufacturers. Blackwell Publishing Ltd 2009-07-13 2009-09 /pmc/articles/PMC4940803/ /pubmed/21462401 http://dx.doi.org/10.1111/j.1750-2659.2009.00095.x Text en © 2009 Blackwell Publishing Ltd |
spellingShingle | Original Articles Li, Yunsheng Larrimer, Audrey Curtiss, Teresa Kim, Jaekyung Jones, Abby Baird‐Tomlinson, Heather Pekosz, Andrew Olivo, Paul D. Influenza virus assays based on virus‐inducible reporter cell lines |
title | Influenza virus assays based on virus‐inducible reporter cell lines |
title_full | Influenza virus assays based on virus‐inducible reporter cell lines |
title_fullStr | Influenza virus assays based on virus‐inducible reporter cell lines |
title_full_unstemmed | Influenza virus assays based on virus‐inducible reporter cell lines |
title_short | Influenza virus assays based on virus‐inducible reporter cell lines |
title_sort | influenza virus assays based on virus‐inducible reporter cell lines |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940803/ https://www.ncbi.nlm.nih.gov/pubmed/21462401 http://dx.doi.org/10.1111/j.1750-2659.2009.00095.x |
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