A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples

BACKGROUND: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimias...

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Autores principales: Qiu, Xu Guang, Nakamura-Uchiyama, Fukumi, Nawa, Yukifumi, Itoh, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940920/
https://www.ncbi.nlm.nih.gov/pubmed/27433138
http://dx.doi.org/10.1186/s41182-016-0019-4
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author Qiu, Xu Guang
Nakamura-Uchiyama, Fukumi
Nawa, Yukifumi
Itoh, Makoto
author_facet Qiu, Xu Guang
Nakamura-Uchiyama, Fukumi
Nawa, Yukifumi
Itoh, Makoto
author_sort Qiu, Xu Guang
collection PubMed
description BACKGROUND: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed. RESULTS: Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples. Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %. CONCLUSIONS: The ELISA with urine samples, which are collected safely and easily, will be a useful tool for a mass-screening of paragonimiasis.
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spelling pubmed-49409202016-07-18 A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples Qiu, Xu Guang Nakamura-Uchiyama, Fukumi Nawa, Yukifumi Itoh, Makoto Trop Med Health Research BACKGROUND: Paragonimiasis is one of the foodborn trematodiases and number of the patients was estimated to be about 23 million around the world. To obtain good compliance of people for the surveillance of paragonimiasis, an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of paragonimiasis with unconcentrated urine samples was developed. RESULTS: Paragonimus westermani antigen specific IgG and IgG4 were detected in urine samples from paragonimiasis patients and the levels correlated well with those detected in the paired serum samples. Cross-reactions observed among other trematodiasis and a tuberculosis patient with the antigen specific IgG were much reduced by detecting the antigen specific IgG4; 9.2 % to 2.3 %. CONCLUSIONS: The ELISA with urine samples, which are collected safely and easily, will be a useful tool for a mass-screening of paragonimiasis. BioMed Central 2016-06-29 /pmc/articles/PMC4940920/ /pubmed/27433138 http://dx.doi.org/10.1186/s41182-016-0019-4 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Qiu, Xu Guang
Nakamura-Uchiyama, Fukumi
Nawa, Yukifumi
Itoh, Makoto
A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
title A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
title_full A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
title_fullStr A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
title_full_unstemmed A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
title_short A tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
title_sort tool for mass-screening of paragonimiasis: an enzyme-linked immunosorbent assay with urine samples
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940920/
https://www.ncbi.nlm.nih.gov/pubmed/27433138
http://dx.doi.org/10.1186/s41182-016-0019-4
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