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A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiol...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941405/ https://www.ncbi.nlm.nih.gov/pubmed/27404346 http://dx.doi.org/10.1038/srep29278 |
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author | Draghi, W. O. Del Papa, M. F. Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, M. J. Lagares, A. Salas, M. E. López, J. L. Albicoro, F. J. Nilsson, J. F. Torres Tejerizo, G. A. Luna, M. F. Pistorio, M. Boiardi, J. L. Pühler, A. Weidner, S. Niehaus, K. Lagares, A. |
author_facet | Draghi, W. O. Del Papa, M. F. Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, M. J. Lagares, A. Salas, M. E. López, J. L. Albicoro, F. J. Nilsson, J. F. Torres Tejerizo, G. A. Luna, M. F. Pistorio, M. Boiardi, J. L. Pühler, A. Weidner, S. Niehaus, K. Lagares, A. |
author_sort | Draghi, W. O. |
collection | PubMed |
description | Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium Sinorhizobium meliloti growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that S. meliloti stopped growing at ca. pH 6.0–6.1. Under such stress the rhizobia increased the O(2) consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia. |
format | Online Article Text |
id | pubmed-4941405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49414052016-07-20 A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti Draghi, W. O. Del Papa, M. F. Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, M. J. Lagares, A. Salas, M. E. López, J. L. Albicoro, F. J. Nilsson, J. F. Torres Tejerizo, G. A. Luna, M. F. Pistorio, M. Boiardi, J. L. Pühler, A. Weidner, S. Niehaus, K. Lagares, A. Sci Rep Article Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium Sinorhizobium meliloti growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that S. meliloti stopped growing at ca. pH 6.0–6.1. Under such stress the rhizobia increased the O(2) consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia. Nature Publishing Group 2016-07-11 /pmc/articles/PMC4941405/ /pubmed/27404346 http://dx.doi.org/10.1038/srep29278 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Draghi, W. O. Del Papa, M. F. Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, M. J. Lagares, A. Salas, M. E. López, J. L. Albicoro, F. J. Nilsson, J. F. Torres Tejerizo, G. A. Luna, M. F. Pistorio, M. Boiardi, J. L. Pühler, A. Weidner, S. Niehaus, K. Lagares, A. A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti |
title | A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti |
title_full | A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti |
title_fullStr | A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti |
title_full_unstemmed | A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti |
title_short | A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti |
title_sort | consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium sinorhizobium meliloti |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941405/ https://www.ncbi.nlm.nih.gov/pubmed/27404346 http://dx.doi.org/10.1038/srep29278 |
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