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Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations

Endothelial cells (ECs) are continuously exposed in vivo to cyclic strain and shear stress from pulsatile blood flow. When these stimuli are applied in vitro, ECs adopt an appearance resembling their in vivo state, most apparent in their alignment (perpendicular to uniaxial strain and along the flow...

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Autores principales: Sinha, Ravi, Le Gac, Séverine, Verdonschot, Nico, van den Berg, Albert, Koopman, Bart, Rouwkema, Jeroen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941569/
https://www.ncbi.nlm.nih.gov/pubmed/27404382
http://dx.doi.org/10.1038/srep29510
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author Sinha, Ravi
Le Gac, Séverine
Verdonschot, Nico
van den Berg, Albert
Koopman, Bart
Rouwkema, Jeroen
author_facet Sinha, Ravi
Le Gac, Séverine
Verdonschot, Nico
van den Berg, Albert
Koopman, Bart
Rouwkema, Jeroen
author_sort Sinha, Ravi
collection PubMed
description Endothelial cells (ECs) are continuously exposed in vivo to cyclic strain and shear stress from pulsatile blood flow. When these stimuli are applied in vitro, ECs adopt an appearance resembling their in vivo state, most apparent in their alignment (perpendicular to uniaxial strain and along the flow). Uniaxial strain and flow perpendicular to the strain, used in most in vitro studies, only represent the in vivo conditions in straight parts of vessels. The conditions present over large fractions of the vasculature can be better represented by anisotropic biaxial strains at various orientations to flow. To emulate these biological complexities in vitro, we have developed a medium-throughput device to screen for the effects on cells of variously oriented anisotropic biaxial strains and flow combinations. Upon the application of only strains for 24 h, ECs (HUVECs) aligned perpendicular to the maximum principal strain and the alignment was stronger for a higher maximum:minimum principal strain ratio. A 0.55 Pa shear stress, when applied alone or with strain for 24 h, caused cells to align along the flow. Studying EC response to such combined physiological mechanical stimuli was not possible with existing platforms and to our best knowledge, has not been reported before.
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spelling pubmed-49415692016-07-20 Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations Sinha, Ravi Le Gac, Séverine Verdonschot, Nico van den Berg, Albert Koopman, Bart Rouwkema, Jeroen Sci Rep Article Endothelial cells (ECs) are continuously exposed in vivo to cyclic strain and shear stress from pulsatile blood flow. When these stimuli are applied in vitro, ECs adopt an appearance resembling their in vivo state, most apparent in their alignment (perpendicular to uniaxial strain and along the flow). Uniaxial strain and flow perpendicular to the strain, used in most in vitro studies, only represent the in vivo conditions in straight parts of vessels. The conditions present over large fractions of the vasculature can be better represented by anisotropic biaxial strains at various orientations to flow. To emulate these biological complexities in vitro, we have developed a medium-throughput device to screen for the effects on cells of variously oriented anisotropic biaxial strains and flow combinations. Upon the application of only strains for 24 h, ECs (HUVECs) aligned perpendicular to the maximum principal strain and the alignment was stronger for a higher maximum:minimum principal strain ratio. A 0.55 Pa shear stress, when applied alone or with strain for 24 h, caused cells to align along the flow. Studying EC response to such combined physiological mechanical stimuli was not possible with existing platforms and to our best knowledge, has not been reported before. Nature Publishing Group 2016-07-12 /pmc/articles/PMC4941569/ /pubmed/27404382 http://dx.doi.org/10.1038/srep29510 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sinha, Ravi
Le Gac, Séverine
Verdonschot, Nico
van den Berg, Albert
Koopman, Bart
Rouwkema, Jeroen
Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
title Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
title_full Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
title_fullStr Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
title_full_unstemmed Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
title_short Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
title_sort endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941569/
https://www.ncbi.nlm.nih.gov/pubmed/27404382
http://dx.doi.org/10.1038/srep29510
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